How can you separate two substances with a Rf(rate of flow) value difference of less than 0.05 in the TLC?
How can you separate two substances with a Rf(rate of
flow) value difference of less than 0.05 in the TLC?
Homework Answers
The substances with a Rf value difference of less than 0.05 in the TLC can be Separated by decreasing the polarity of mobile phase. With the decrease in the polarity of mobile phase, the difference in the binding ability of substances on the mobile phase increases and hence they can be easily separated.
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How can you separate two substances with a Rf(rate of flow) value difference of less than 0.05 in the TLC?
Determine the Rf value of the substances A, B, and C on the TLC shown. Show...
Determine the Rf value of the substances A, B, and C on the TLC
shown. Show your calculation!
What will happen if the applied spot is lower than the level of
the developing liquid?
What will be the result of applying too much compound on a TLC
plate?
What is the reason that the mobile phase travels up the silica
gel plate (TLC plate)?
4. Determine the Rf value of the substances A, B, and Con the TLC shown. Show...
How is the Rf value for a spot on a TLC plate calculated? What can the...
How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for?
why can the Rf value be used to distinguish the substances from one another? and Why...
why can the Rf value be used to distinguish the substances from one another? and Why is anRf value rather than the distance the spot moved used to help identify a substance by TLC?
Can an Rf value be less than 0? Can it be greater than 1? Why or...
Can an Rf value be less than 0? Can it be greater than 1? Why or why not?
1. a) Why does biphenyl have a higher Rf value than either benzophenone or triphenylmethanol? Exp...
1. a) Why does biphenyl have a higher Rf value than either benzophenone or triphenylmethanol? Explain based on the structures and their effect on mobility in the TLC process. b) Which compound, benzophenone or triphenylmethanol has a higher Rf value? Explain, based on their structures, how you can predict this. c) You developed your TLC using a 10% mixture of diethyl ether in petroleum ether. Petroleum ether is a mixture of non-polar alkanes, so this TLC solvent is composed of...
2) During silica gel TLC analysis, when 2-propanol was used as a developing solvent, two substances...
2) During silica gel TLC analysis, when 2-propanol was used as a developing solvent, two substances moved with the solvent front (Rf = 1.0). Can you conclude that the substances are identical? If not, what additional experiment(s) would you perform? (5 pts).
When 2-propanol was used as the developing solvent, two substances moved with the solvent front (Rf...
When 2-propanol was used as the developing solvent, two substances moved with the solvent front (Rf = 1) during TLC analysis on a silica gel plate. Can you conclude that they are identical? If not, what additional experiments(s) would you perform?
1. When 2-propanol was used as the developing solvent, two substances moved with the solvent front...
1. When 2-propanol was used as the developing solvent, two substances moved with the solvent front (Rf= 1) during TLC analysis on a silica gel plate. Can you conclude that they are identical? If not, what additional experiment(s) would you perform? 2. The Rf value of compound A is 0.34 when a TLC plate is developed in hexane and 0.44 when the plate is developed in diethyl ether. Compound B has an Rf value of 0.42 in hexane and 0.60...
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front...
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm. 2) A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the unknown material a pure compound? What can be done to verify the purity of the sample using thin-layer chromatography? 3) You try to synthesize n-hexyl bromide as a product...
Bonferroni's method ensures that the family-wise error rate is less than or equal to the design...
Bonferroni's method ensures that the family-wise error rate is less than or equal to the design level such as 0.05 after all possible pairs-wise comparisons have been made. Three groups have 3 possible pairs-wise comparisons. Four groups have 6 possible pairs-wise comparisons. Please answer following questions 26-29 based on this method. A study has three groups. Given an overall design error rate 0.05, a pairs-wise comparison can be claimed statistically significant difference when the p-value less than Group of answer...
Determine the Rf value of the substances A, B, and C on the TLC
shown. Show your calculation!
What will happen if the applied spot is lower than the level of
the developing liquid?
What will be the result of applying too much compound on a TLC
plate?
What is the reason that the mobile phase travels up the silica
gel plate (TLC plate)?
4. Determine the Rf value of the substances A, B, and Con the TLC shown. Show...
2) During silica gel TLC analysis, when 2-propanol was used as a developing solvent, two substances moved with the solvent front (Rf = 1.0). Can you conclude that the substances are identical? If not, what additional experiment(s) would you perform? (5 pts).
1. When 2-propanol was used as the developing solvent, two substances moved with the solvent front (Rf= 1) during TLC analysis on a silica gel plate. Can you conclude that they are identical? If not, what additional experiment(s) would you perform? 2. The Rf value of compound A is 0.34 when a TLC plate is developed in hexane and 0.44 when the plate is developed in diethyl ether. Compound B has an Rf value of 0.42 in hexane and 0.60...
1) Calculate the Rf value of a spot that travels 5.7 cm, with a solvent front that travels 13 cm. 2) A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the unknown material a pure compound? What can be done to verify the purity of the sample using thin-layer chromatography? 3) You try to synthesize n-hexyl bromide as a product...
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