Question

biochemistry

5) Bisphenol A is used to synthesis a lot of different polymers used in consumer goods. Bisphenol A is reported to have estrogenic activity and is found to be a problematic environmental contaminant due to its effect(s) on biological organisms. Design/describe an experiment in which the activities of bisphenol A and estradiol are compared. a 6) You are working in a lab and generate a monoclonal antibody that recognizes phosphotyrosine. How would that Ab affect insulin signaling?

Answer 1

Ans- Evidence exists that raices concern about genotoxic. Bisphenol A (BPA) a monomers of plastic used in consumer product, is abu andant inenvironment and entors the body by ingestion or adsorption We develop a cell based transcription assaysystem using a reporter gene unden the transcriptional control of estrogen receptor alphalt Ralpha) as well as Erbela and performed chloramphenicol. acetyltransferase (CAT) assay on Hela cells transfected with either human & Ralpha EDNA. and Erbeta CDNA to characterize the estrogenic effect of BPA

bXMetA, which mimicked the glucoregulatorn but not the mitogenic, action of insulin. Biophysical studies with cultured cellsexpressing human INSR demonstrated that XMet A acted allosterically and did not complete with insulin. for binding to its receptor.