Question

The following is absorption (A) and fluorescence (F) spectra of spinach chloroplasts. Draw an energy level diagram for the chromophore showing transitions with arrows for the peaks at 730 nm, 683 nm, 678 nm, 625 nm, and 440 nm. Label the states. The peaks of absorption and emission bands are at slightly different wavelengths, why? 2. r -1.5 1.5 6787r683 -1.0 1.0H A H0.5군 0.5 F 0.0 400 Jo.0 800 600 700 WAVELENGTH (nm) 500 igure 1. Absorption (A) and fluores cence (F) spectra of spinach chloroplasts ABSORBANCE oi FL INTENSITY

Answer 1

Sol Enes3y Level diaJsam - In absosption (A) and Fiusosconse (F) SPecta of sPinach chiosoplasts S3 2.88ev (440nm) vibšation a Repatia 198e (625 nm) S2 82 C (618nm) C683nm) a18 1 S, 69 ev (130nm) So Groound state absosbtion So iom S2 C doun When elated enesgy level leve Reiated to to S2 leveL ihe enesgy leveL by vibsatio n C Comes down to jvound (So) elanation Joes to cs Scanned with CamScanner