Question

1: What happens in a molecular level that allows an analyze to absorb light at a certain wavelength.

2: Why are you using a specific wavelength of light to record absorbance measurements for the dye (i.e., why non't every wavelength of light be absorbed by the analyte)

I need this answer ASAP please.

Thank you

Answer 1

1. when sample molecules are exposed to light having an energy that matches a possible electronic transition within the molecule, some of the light energy will be absorbed as the electron is promoted to higher energy orbital.

2.because different compounds absorbs light at different wavelengths,

and why different compounds absorbs different wavelength of light because because each bond in a perticular compond is different each will vibrate in different way and absorbs different wavelengths.