Homework Answers
A longer column will produce longer retention times but better separation. The length of a column affects the retention time proportionally. Thus halving the column length will reduce the retention time to half as well. But the quality of the separation will go down as well. Reducing retention time has many benefits such as saving time, chemicals, and resources etc.
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If a column is exchanged for a similar column that is half the length of the...
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed...
A GC column is operated under the following conditions: Column: 1.10 m x 2 mm packed with Chromosorb P and Carbowax 20M as stationary phase. The following retention times were obtained: air: 18.0 sec; methylacetate: 2.98 min; methyl propionate: 4.56 min. Peak width at half height: methylacetate: 7.7 sec; methyl propionate: 12.4 sec. Calculate: a) the corrected retention time for each solute. b) the capacity factor k' for each solute. c) the selectivity factor α for the pair of solutes....
If you were to use a column twice the length of the one that you will...
If you were to use a column twice the length of the one that you will actually use in lab, how do you think the retention times of the compounds would be affected? How do you think the seperation of the peaks would be affected? Please answer & explain.
Two solutes are separated on a column. Solute A has a shorter retention time than Solute...
Two solutes are separated on a column. Solute A has a shorter retention time than Solute B. Explain the relative attraction of Solutes A & B to the mobile and stationary phases.
An open tubular column with a diameter of 193 um and a stationary phase thickness on...
An open tubular column with a diameter of 193 um and a stationary phase thickness on the inner wall of 0.60 um passes unretained solute through in 57 s. A particular solute has a retention time of 485 s. What is the partition coefficient for this solute? K= What fraction of time does this solute spend in the stationary phase?
What length of column would be required to just resolve two peaks with retention times of...
What length of column would be required to just resolve two peaks with retention times of 92 and 107 s, respectively, using a column with an Hof 1.60 cm/plate?
An open tubular column with a diameter of 187 um and a stationary phase thickness on...
An open tubular column with a diameter of 187 um and a stationary phase thickness on the inner wall of 0.88 um passes unretained solute through in 79 s. A particular solute has a retention time of 471 s (b) What fraction of time does this solute spend in the stationary phase? (a) What is the partition coefficient for this solute? Number Number 259.8 4.96 K= total
A solute with a retention time of 4 mins 25 second has a base width of...
A solute with a retention time of 4 mins 25 second has a base width of 12 seconds. The column is 30.0m long. How many theoretical plates does the column have? What is length in mm of one theoretical plate?
In gas chromatography, when two compunds have very similar retention times, explain what change could be...
In gas chromatography, when two compunds have very similar retention times, explain what change could be made to 1. pressure 2 heating ramp rate and 3 length of column to achieve a better separation. thankyou!
A solvent passes through a chromatography column in 2.85 min, but the solute requires 9.43 min....
A solvent passes through a chromatography column in 2.85 min, but the solute requires 9.43 min. What is the retention factor, k?k= What fraction of the time does the solute spend in the mobile phase in the column? tfraction in mobile phase= The volume of the stationary phase is 0.115 times the volume of the mobile phase in the column (Vs=0.115Vm). What is the partition coefficient, K, for this system? K=
Two compounds C and D are separated on a column 34 cm long . Given the...
Two compounds C and D are separated on a column 34 cm long . Given the chromatographic data below calculate The column resolution? , the number of theoretical plates ?the height of theoretical plate and what length of column would be required to give a resolution of 1.5? Compound C retention time 18.3 width at base of peak(min) = 2.18 Compound D retention time 17.7, width at base 1.23
An open tubular column with a diameter of 193 um and a stationary phase thickness on the inner wall of 0.60 um passes unretained solute through in 57 s. A particular solute has a retention time of 485 s. What is the partition coefficient for this solute? K= What fraction of time does this solute spend in the stationary phase?
An open tubular column with a diameter of 187 um and a stationary phase thickness on the inner wall of 0.88 um passes unretained solute through in 79 s. A particular solute has a retention time of 471 s (b) What fraction of time does this solute spend in the stationary phase? (a) What is the partition coefficient for this solute? Number Number 259.8 4.96 K= total
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