Question

1. What is the purpose of “Boc anhydride,” (t-BUOCO)20, in the synthesis of polypeptides? O To protect the amino nitrogen in the amino acid acting as electrophile. O To activate the amino nitrogen in the amino acid acting as nucleophile. O To activate the carboxylic acid (i.e., turn -OH into a good leaving group) in the amino acid acting as electrophile. O To protect the carboxylic acid in the amino acid acting as nucleophile. O To cleave the nitrogen protecting group at the N-terminus at the end of synthesis.

Answer 1

1 What is the purpose of 'Boc anhydxide, Ct-Buoco), in the synthesis of polypeptides? Amas' To protect the amino nitrogen in the anino acid acting as electrophile. • Boc anhydride reacts with anine to give N-tert- butony carebonył o Boc derivatives. This derivative does not behave as amines. This Boc group can later be removed from the amine facer using moderately strong acids (Cfg cooh ). & Boc serves as protective group in solid phase peptide Synthesis