Question

According to the chromatogram 4.51 5.4 6.85 Detector response + 8.3 6 10 20 30 20 6.0 46.0 47.0 2.0A se 10 20 0 Time (min) a. Knowing that the partition coefficient for solute B is 40.0, what is the partition coefficient for solute A? (2.5 pts)

Answer 1

The partition coefficient for solute A can be determined by correlating the retention time of solute B in the chromatogram.

For solute B = Retention time = 7.5 minutes and partition coefficient = 40

Therefore, Partion coefficient for solute A with retention time of 5 minutes = 5 x 40/7.5 = 26.6