Beef catalase has been used to accelerate the decomposition of hydrogen peroxide to yield water and oxygen [Cheni. Eng. Ecluc., 5, 141 (1971)]. The concentration of hydrogen peroxide is given as a function of time for a reaction mixture with a pH of 6.76 maintained at 30 °C.
1 (min) | 0 | 10 | 20 | 50 | 100 |
Ch2o2 (mol/L) | 0.02 | 0.01775 | 0.0158 | 0.0106 | 0.005 |
(a) Determine the Michaelis−Menten parameters Vmax and KM.
(b) If the total enzyme concentration is tripled, what will the substrate concentration be after 20 minutes?
(c) How could you make this problem more difficult?
(d) List ways you can work this problem incorrectly.
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