![y = 10.204x - 0.7485 1/Initial Rate (min/umole) Vertical (Value) Axis Major Gridlines 68.289 0 10 20 30 40 1/ [ONPG] (mM-1) 5](http://img.homeworklib.com/questions/ee8dbdc0-704c-11ea-ad02-ad9ab96d4cbe.png?x-oss-process=image/resize,w_560)
Please calculate the Km and VMax for each enzyme(E.coli and A.oryzae) for ortho-Nitrophenyl-β-galactoside
Homework Answers
The graphic presented is that of Lineweaver Burk, you have:
b = 1 / Vmax = 0.7485
Vmax is cleared = 1,336
It has:
m = Km / Vmax = Km / 1,336 = 10,204
Km = 13,633 is cleared
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Please calculate the Km and VMax for each
enzyme(E.coli and A.oryzae) for ortho-Nitrophenyl-β-galactoside
y = 10.204x...
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed react...
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The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. S Where v is the velocity or rate, Vmax is the maximum velocity, Km is the +IST Michaelis- Menten constant, and I5 s the substrate concentration. K + S v (uM/min) a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (Vo) at different substrate concentrations ([S]) 300 Vmax 250 1/2 Vmax First, move the line labeled "Vmax to a...
a. what are the values of Vmax and Km in the abscence if the
inhibitor what are the values of Vmax and Km in the presence of the
inhibitor?
b. what type of inhibition is it?
c. what is the dissociation constant (Ki) of the
inhibition?
***d. graph a linear scatter plot including equation.
Homework (CHE 407) The initial velocity for an enzyme-catalyzed reaction is measured at various initial substrate concentration [S]o, in the absence and in the presence of...
Based on the document below,
1. Describe the hypothesis Chaudhuri et al ids attempting to
evaluate; in other words, what is the goal of this paper? Why is he
writing it?
2. Does the data presented in the paper support the hypothesis
stated in the introduction? Explain.
3.According to Chaudhuri, what is the potential role of thew
alkaline phosphatase in the cleanup of industrial waste.
CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10,...
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