Question

Based on the document below,

1. Describe the hypothesis Chaudhuri et al ids attempting to evaluate; in other words, what is the goal of this paper? Why is he writing it?

2. Does the data presented in the paper support the hypothesis stated in the introduction? Explain.

3.According to Chaudhuri, what is the potential role of thew alkaline phosphatase in the cleanup of industrial waste.

CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 8.5, 9, 9.5, 10, 10.5, 11, 11.5 and 12). In a typical rea

INDIAN J. BIOCHEM. BIOPHYS., VOL. 50, FEBRUARY 2013 investigated. The effects of pH on the kinetics of CIAP-catalyzed PNPP hy

CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP . ex (umoles min unit) 0.007 0.006 0.005 pH 8.5-12 in Tri

INDIAN J. BIOCHEM. BIOPHYS., VOL. 50, FEBRUARY 2013 Vox (umoles murunit) (b) 0.0018 0.0016 0.0014 0.0012 0.001 0.0008 0.0006

CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 2.51(a) Enzyme activity (moles min unit) Enzyme activity

INDIAN J. BIOCHEM. BIOPHYS., VOL. 50, FEBRUARY 2013 ---Cu Cu 50 ppm initial cone, & pl 11 100 ppm initial conc. & pH11 Precip

CHAUDHURI et al: KINETIC BEHAVIOUR OF CALF INTESTINAL ALP WITH PNPP 71 5 McCarthy AD, Cortizo A M, Segura G G, Bruzzone L & E

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Answer #1

1.report the kinetic behaviour This paper of CIAP (active ALP) with p-nitrophenyl phosphate and the effects of pH, buffer Subst

2.The study came to the conclusion that the enzyme at different conditions(pH,Buffer,substrate concentration) it undergo some conformational changes leading to the changes in the cataalytic activity.These changes can be analysedand CIAP can precipitate heavy metals used for industrial purposes.Thus the data in the paper supports the hypothesis

3.CIAP can precipitate Cu2+.It has the ability of inorganic phosphate driven precipitation catalysed by CIAP.This effect can be used for the clean up of industrial waste.

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