Question

A HPLC column was operated under the following conditions

Column Length – 25.4 cm      Column Diameter = 0.5 mm

Mass stationary phase = 0.49 g          Density Stationary Phase = 1.12 g/mL

Measured Outlet Flow Rate = 1.0 mL/min

Column Temperature = 25 ^oC.

resolution equation=R_s = Trb-Tra/W

Plate height(H) = length of column (L)/Number of plate on the column (N)

No. of theoretical N= 16(R_S/w)²

Calculate the resolution for components #3 and #4?

Calculate the plate height for column (use component #3?

Calculate the number of theoretical plates(use component #3?

0 1 2 3 4 8 9 10 5 6 7 Time (min)

Answer 1

The resolution for components #3 = (2.9 - 1.2)/w

Note: Here, the width at half-height (w) values are not given, actually, if the peak length is given, then half-of the peak length itself is the value of 'w'.

The resolution for components #4 = (4.1 - 1.2)/w

Note: Here also, the width at half-height (w) values are not given, actually, if the peak length is given, then half-of the peak length itself is the value of 'w'.