Question

t How are donaturation and varovalo airterent a. Rydrolysis resulte to the formation of water. Hydrolysis only takes place in the cytopa c. Hydrolysis results in breakingole d. Hydrolysis results in breaking ovalent bo How is folding related to function? • Folding and function are not related.. Proper folding results in propertune 0. Proper folding results in reduced un roper folding results in arratiefunct Molecules that a t folding are called a. Foldons which cellular environment is . Cytoplasm c. Adipose d b. Proteases 25. A protein is active in all a) proteins are more 11 26. 0. Chaperon d. Transports onment is least l y to harbor nonpolat proteins? b. Membrane d. Mitochondrial Terbane Dove 5. h a ve in acide but quickly loses its activity at pH values decrease in activity is probably because b) ename more likely to be protonated in acide solution. el at a higher unction less efficiently in neutral solutions. d) alph functional groups hydrolyze alpha helical regions are taie in acidic solutions. Watch the chromatographite technique with its separation basis. a) Affinity chromatography _1) Charge b) Thin layer chromatography 2) Size c) Gel Filtration chromatography _3) Solubility d) Ion-exchange chromatography _4) Molecular Bonding What reaction is achieved by treating proteins with B-mercaptoethanol? a. Hydrolysis of peptide bonds. b. Hydrolysis of disulfide bonds. c. Hydrolysis of N-terminal amino acids. d. Hydrolysis of C-terminal amino acids. Identify any proteolytic protein (protease). a. Trypsin b. Cytochrome C c. RNAse d. CNB Two different schemes (schemes A and B) were used to purify a protein. The total and specific activity for scheme A is 20.0 and 12.0 respectively. The total and specific activity for scheme Bis 20.0 and 8.0 respectively. Which has the higher concentration of activity? a. Scheme A b. Scheme B c. Neither (they're the same) A peptide was sequenced and the sequence of fragment sets generated by different cleavage methods is shown below. What is the primary structure of the peptide? Trypsin: Chymotrypsin: Staph protease: AIIALNGASTIR + MGGAQILDIGCFEK + AGAGG EKAIIAL + NGASTIRAGAGG + MGGAQIL + DIGCE MGGAQILD + KAIIALNGASTIRAGAGG + IGCFE 1. EKAIIALNGASTIRAGAGGMGGAQILDIGCF • AGAGGAIIALNGASTIRMGGAQILDIGCFEK b. AIIALNGASTIRMGGAQILDIGCFEKAGAGG d. MGGAQILDIGCFEKAIIALNGASTIRAGAGG

Answer 1

21) Answer is d). Hydrolysis involve breaking of covalent bond and split the molecule into two products.

22) Answer is b). Proper folding leads to proper structure and shape and thus proper function.

23) Answer is c). Chaperone perform folding of proteins.