For an enzyme that follows the Michaelis-Menten kinetic, what substrate concentrations (relative to Km) are needed...
For an enzyme that follows the Michaelis-Menten kinetic, what substrate concentrations (relative to Km) are needed for the speed of the reaction to be
- 0.12 there vmax
- 0.25 there vmax
- 0.5 there vmax
- 0.9 there vmax.
Homework Answers
![solution The michaelis-menten equation is given by ZZZZZZLL2222 Umax [s] 7= kent [s] re speed of reaction Vmax= maximum rate.](http://img.homeworklib.com/questions/7ca6cc20-71db-11ea-bb0d-b3321e412197.png?x-oss-process=image/resize,w_560)
![km X0 12 = 0.88* [s] = 0.12 kmat - 0.136 km 10.88 [s] = D.186 km And r - 0.25 Vmax Hence, 0.25 = 1ST Kent [s] 0.25km +0.25 [s](http://img.homeworklib.com/questions/7d88f500-71db-11ea-a1dc-273ffc67ffeb.png?x-oss-process=image/resize,w_560)
![HIFFHHHHHFFFFFFFFFFEE (C) = 0.5 U max [s] Hence, 0.5= kon + [s] 0.5x kas & 0.5 [s] = [s] 0.5 ks = 0.5 [s [s] = km Ane (d) r=](http://img.homeworklib.com/questions/7e634660-71db-11ea-97cb-7ff1426f3676.png?x-oss-process=image/resize,w_560)
Add Answer to:
For an enzyme that follows the
Michaelis-Menten kinetic, what substrate concentrations (relative
to Km) are needed...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate ...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed react...
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. S Where v is the velocity or rate, Vmax is the maximum velocity, Km is the +IST Michaelis- Menten constant, and I5 s the substrate concentration. K + S v (uM/min) a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (Vo) at different substrate concentrations ([S]) 300 Vmax 250 1/2 Vmax First, move the line labeled "Vmax to a...
1. Michaelis and Menten examined how the velocity of enzyme catalyzed reactions change with substrate concentration....
1. Michaelis and Menten examined how the velocity of enzyme catalyzed reactions change with substrate concentration. Which of the following is (are) common to all enzyme catalyzed reactions? Velocity is insensitive to changes in [substrate] at all substrate concentrations. Km is the [substrate] required to reach 50% of Vmax. Velocity is responsive to changes in [substrate] when the Km > [substrate]. Velocity is insensitive to [substrate] when [substrate] is much greater than Km. Velocity reaches 90% of Vmax when [substrate]...
The relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation a) At what substrate concentration would...
The relation between Reaction Velocity and Substrate Concentration: Michaelis-Menten Equation a) At what substrate concentration would an enzyme with a kcat of 30.0 s-1 and a Km of 0.0050 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations: [S]=Km/2, [S]=2Km, [S]=10Km
An enzyme follows Michaelis-Menten kinetics. Indicate (with an "x") which of the kinetic parameters would be...
An enzyme follows Michaelis-Menten kinetics. Indicate (with an "x") which of the kinetic parameters would be altered in the presence of the corresponding type of inhibitors. Inhibitor type Vmax Km Neither Both Competitive Uncompetitive Noncompetitive
For an enzyme that displays Michaelis-Menten kinetics, what is the reaction velocity v (as a percentage...
For an enzyme that displays Michaelis-Menten kinetics, what is the reaction velocity v (as a percentage of Vmax) ,observed at each of the following substrate concentrations. (Ex, v = xVmax, where x = an integer, fraction, or decimal number (two decimal places)) a) [S] = 0.1 Km _________ b) [S] = 2 Km _________ c) [S] = 10 Km _________
What is the velocity of a Michaelis-Menten enzyme reaction (in terms of vmax) when the concentration...
What is the velocity of a Michaelis-Menten enzyme reaction (in terms of vmax) when the concentration of substrate is 4 times the value of KM? Show your work.
3. The Michaelis-Menten Graph also shows the theoretical maximum rate of the enzyme (Vmax), the point...
3. The Michaelis-Menten Graph also shows the theoretical maximum rate of the enzyme (Vmax), the point where the enzyme is working at its maximum rate (Vmax/2), and amount of substrate needed to bind half of the active sites (Km). Label these points on the graph. Vmax represents: Vm Vmax/2 represents: Reaction velocity v Vmax 2 Km represents: Kim Substrate concentration (5)
will rate thanks Q1. WHAT ARE ENZYMES? HOW DOES ENZYME-SUBSTRATE BINDING TAKES PLACE? Q2. IN MICHAELIS...
will rate thanks
Q1. WHAT ARE ENZYMES? HOW DOES ENZYME-SUBSTRATE BINDING TAKES PLACE? Q2. IN MICHAELIS -MENTEN GRAPH, WHY DOES THE CURVE REACHES PLATEAU? Vmax Reaction velocity (v) Vm/2 Km Substrate concentration (S) Q3. IN MICHAELIS MENTEN GRAPH, HOW WOULD YOU INCREASE VELOCITY BEYOND Vmax? Q4. SMALLER VALUE OF THE MICHAELIS CONSTANT (Km) REFLECTS HIGHER EFFICIENCY OF THE ENZYME. (TRUE/FALSE).
An enzyme that follows Michaelis-Menten kinetics has a KM value of 20.0 μM and a kcat...
An enzyme that follows Michaelis-Menten kinetics has a KM value of 20.0 μM and a kcat value of 211 s−1. At an initial enzyme concentration of 0.0100 μM, the initial reaction velocity was found to be 1.07×10−6 μM/s. What was the initial concentration of the substrate, [S], used in the reaction ? Express your answer in micromolar to three significant figures.
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
The Michaelis-Menten equation is often used to describe the kinetic characteristics of an enzyme-catalyzed reaction. S Where v is the velocity or rate, Vmax is the maximum velocity, Km is the +IST Michaelis- Menten constant, and I5 s the substrate concentration. K + S v (uM/min) a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (Vo) at different substrate concentrations ([S]) 300 Vmax 250 1/2 Vmax First, move the line labeled "Vmax to a...
3. The Michaelis-Menten Graph also shows the theoretical maximum rate of the enzyme (Vmax), the point where the enzyme is working at its maximum rate (Vmax/2), and amount of substrate needed to bind half of the active sites (Km). Label these points on the graph. Vmax represents: Vm Vmax/2 represents: Reaction velocity v Vmax 2 Km represents: Kim Substrate concentration (5)
will rate thanks
Q1. WHAT ARE ENZYMES? HOW DOES ENZYME-SUBSTRATE BINDING TAKES PLACE? Q2. IN MICHAELIS -MENTEN GRAPH, WHY DOES THE CURVE REACHES PLATEAU? Vmax Reaction velocity (v) Vm/2 Km Substrate concentration (S) Q3. IN MICHAELIS MENTEN GRAPH, HOW WOULD YOU INCREASE VELOCITY BEYOND Vmax? Q4. SMALLER VALUE OF THE MICHAELIS CONSTANT (Km) REFLECTS HIGHER EFFICIENCY OF THE ENZYME. (TRUE/FALSE).
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 10 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 10 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 10 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 10 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 10 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 10 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 10 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 10 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 10 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 10 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 10 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 10 months ago