Question

Prepare at least 6 dilutions of the protein standard, with concentrations of 4, 8, 12, 16, 20, and 24 µg/ml. Do this by diluting pre-calculated volumes of the BSA standard (2 mg/ml) into volumes of distilled water, which results in a final volume of 1.0 ml.

Please show calculations for all 6 dilutions.

Answer 1

2000 stock Solution zmaIme = 2000 ug/ml [img=1000 mg] 1.Preparation of Higimb sobilia Vstock X Estock = V4 X CA IVA=Volume of Auglmi som = imeo Vstock X 2000 lug/mL)= 1 ml ! CA = Augline 1 x 4 lug/mL) op Vstock = 1*4 ml =0.002m2 = 2 let 2. Preparation of 8 felg/m) solo Vstock X 2000 fugime) = 1 mL x 8 (regime) op Vstock = 1.80 ml = 0 coume = 4 eet . 3. Preparation of 12 (leglm) som Vstock x 2000 (Mgmt.) = 1 ml * 12 fuglme) op Vstock = 1812 mb = !*12*1000 let = Gelb 4. Preparation of is egime) som Vstock X 2000 leegime) = 1 ml x 16 lug/mL) i op Vstock = 1x 16 mb = 164210000 LL = 861 5. Preparation of 20 (eglamh) som o Vstock x 2000 (ugimi) = 4. mk * 20 (regime) or Vstock = bxo ml = (20x1000) L = loub 6. Preparation of 24 feg/ml) som Vstock X 2000 (ug/mL) = 1 ml x 24 luglue) or Vstock' = 424 mb = 24X1000 ML = 12 let 200 2000 2000 2000 2000