Question

ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work. 2. If the original BSA (albumin) stock solution had been made incorrectly so that it was 2.5 mg/mL instead of 2.0 mg/mL, how would this change the results? Would the concentration of the unknown be underestimated or overestimated? Explain your reasoning 3. In a protein assay, a 75uL sample contained 18 μg of protein. What is the concentration of this sample (in mg/mL)? Show your work. 4. A protein sample is diluted 5-fold before measuring the protein concentration in a protein assay. In the protein assay, the concentration of the diluted protein is determined to be 0.27 mg/mL. What is the concentration of the original protein sample? Show your work

Answer 1