please help with this question .and explain so i can undestand how to do that.thank you...
please check my answer if i did right Making solutions with multiple solutes (Part II) 0.15 molo / 17.582 9 gnad 1. (5 points) Describe how to prepare a 2L solution: 15 mol 250 mM NaCl 58.449/md Y2LX 0.15 m 1% NP-40 (v/ve) 0.01 X 26 2 9 0.1% SDS (w/v), 0.001% 2L 0.0029 / SAS 50 mM Tris-HCI 0.05 X 62.14 = 6.0579 16.057 g o Hell + DI Hgo to a I to o to 2. (4 points)...
Making Solutions Practice Problems: I. How would you prepare 100 ml of a 30% (w/v) solution of polyethylene glycol (PEG)? 2. How would you prepare 50 ml of a 9 % (w /v ) solution of NaCI? 3. How would you prepare 200 ml of 70 % ( v /v ) solution of ethanol from a stock of 95% ethanol? How would you prepare 200 ml of 0.3 M NaCl? (NaCl MW 58.44) 4. 5. How are 50 ml of...
Explain how you would prepare 10 mL of the Laemli Sample Loading Buffer as given below, using the following stock solutions: 1 M DTT, 10% SDS, 1M Tris HCl pH 6.9, 100% Glycerol, 0.2% stock of Bromophenol Blue.
Solutions practice problems. calculate how much of each ingredient you would need to make the solution CIRCLE YOUR FINAL ANSWERS. Be sure to include the correct units with your answer. Molecular weights: SDS-288.38 g/mol Tris (base) = 121.1 g/mol EDTA = 372.2 g/mol Sodium acetate 82.03 g/mol Tris maleate = 237.2 g/mol Magnesium sulfate heptahyrdate=246.5 g/mol Sodium hydroxide - 40.00 g/mol (can calculate the MW for simple compounds) 1) 200 mL of 25% SOS 2) 60 ml alkaline lysis buffer...
7.43 Explain how you would prepare the following solutions us- ing pure solute and water. Assume water has a density of 1.00 g/mL. a. 250 mL of a 2.00 M NaOH solution b. 500 mL of a 40.0% (v/v) alcohol solution (C,H,OH) c.100 mL of a 15.0% (w/v) glycerol solution. Glycerol is a liquid with a density of 1.26 g/mL. Describe two ways to measure out the amount of glycerol needed. d. Approximately 50 mL of a normal saline solution,...
can someone help with #2 150 mL of 10-1-(WA) SDS 1w/veg of solute/100 ml 10g: 100 ml 150 ml 10g) 15g SDS in 150 ml dd Haa (100ml) b. 200 mL of 250mm tris buffer, por 7.5 Tris 1210 g/mol PH 7.5 es pot - 14-7.5 = 6.5 Pka of tris: 205 -> pkb - 14-pka = 5.94 POH pkb + log [ BH"] Balt [B]-* [et] Tel : antilog (6.5-5.94) - 3.631 [4] . 3.631 [8] [BHT] + [b],...
Please I need help with number 54 and 61 please An aqueous NaCl solution is made using 112 g of NaCl diluted to a total solution volume of 1.00 L. Calculate the molarity, molality, and mass percent of the solution. (Assume a density of 1 08 g/mL for the solution.) An aqueous KNO_3 solution is made using 72.5 g of KNO_3 diluted to a total solution volume of 2.00 L. Calculate the molarity, molality, and mass percent of the solution....
help!!! how do I answer this Page 1 of 6 a. (3 Points) If 145 grams of lead nitrate were added to water to make 1.50 x 10 mL of solution, what would be the molarity of the resulting solution Pb(NO) molar mass w 331.21 gmol b. (3 Points) What is the molar concentration of chloride sons in a solution prepared by mixing 50.0 mL of 1.5 M KCI with 100.0 mL of a 2.5 M Cacla solution? took 100%...
You are given a stock solution of ribosylated collagen. In order to complete your experiment, you will need four 1ml aliquots of ribosylated collagen in phosphate buffered saline (1M), each containing 67.5 ng/ml. The stock concentration is 10 ug/ml, What steps are required to achieve the required concentrations? During lab a classmate accidentally breaks an Erlenmeyer flask: a single shard causes a 50mm laceration between his elbow and wrist. You decide that the best way to keep the wound clean...
PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone, 0.5% yeast extract, 10 mM NaCL (F.W. 58), 2.5 mM KCl (F.W. 74.5), 10 mM MgSO4 (F.W. 246.5), 10 mM MgCl2 (F.W. 203.3), 20 mM glucose (F.W. 180.16). Determine the amounts of each to make 500 mL of SOC. 2) Determine the number of moles in 0.05 μg of 300 bp insert DNA. There are 660 g/mol-bp. What is the molar ration of insert...