Question

1. A portion of the stock has to be diluted for this purpose. We could take the required amount and then volume could be made up with Phosphate buffered saline.

10 microgram or 10000ng is present in 1000 microlitre (1ml)

then 67.5 ng will be present in 1000/ 10000 multiplied with 67.5

= 6.75microlitres

6.75microlitres from the stock has to be taken and PBS will be added to make up the volume 1ml.

2. 0.8g of NaCl has to be added to 100ml of sterilized water

3. Suppose, if we start with 100ml of 90% alcohol, we have to tkae 31.5ml of alcohol from it and water could be added to make up the volume to be 100ml, and the resulting solution will be of 30% concentration, i.e we wiill have 100ml of 30% alcohol.

4. for 4 making 4% paraformaldehyde, we have to take 8 grams of paraformaldehyde in 100ml of the buffer.The, glutaraldehyde has to be diluted 10 times from the stock solution with the buffer.

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