Question

J 0 points) The detection limit for caffeine analysis must be calculated. Eight absorbance readings from a caffeine sample close to the estimated detection limit have a mean of 0.1179 The average absorbance for eight blanks is 0.0182 and the standard deviation is 0.005,, The slope of a calibration curve is 0.59 mM. What is the limit of detection? What is the limit of quantitation? Are the caffeine measurements reliable?

Answer 1

Here,

Slope of the calibration curve = 0.59 mM^-1

Standard deviation of the blank measurements = 0.0059

We have,

Limit of detection = (3 x Standard deviation)/Slope

                                = (3 x 0.0059)/ 0.59 mM^-1 = 0.03 mM

Limit of quantification = (10 x Standard deviation)/Slope

                                = (10 x 0.0059)/ 0.59 mM^-1 = 0.1 mM