Describe the common causes of interference in immunoassay
ANS)
Common causes of interference in immunoassay,
The interference is usually caused by the interplay with single or increased number of steps in immunoassay method and analyte concentration or antibody binding is normally affected.
The Interference can enhance or reduce the calculated outcome and may be analyte dependent or analyte independent.
The Analyte dependent interference in immunoassay is resulted by the interplay among constituents in sample with single or increased number of reagent antibodies.The usual interference of lipemia,hemolysis,impact of the anticoagulants are normally independent of analyte concentration.
The Endogenous interfering materials can happen in an healthful and pathological client samples.Every patients or clients have their own individual sample characteristics and interference develop from an interplay with single or increased number of steps in immunoassay method.The quantifiable analyte concentration in specimen or antibody binding is changed by lots of elements.
The Exogenous interference are the interference resulted by initiation of outside factors or situations.The outside factors such as in vivo or in vitro ,that are not usually seen in native,accurately gathered and preserved specimens can initiate interference.
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