Homework Answers
Your positive control should confirm that your target antigen is expressed on the relevant cells and tissues. Your negative control should consist of tissues or cells where your target protein is known to be absent.
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2. AHG reagent is anti-IgG. It is used to detect IgG coated red cells. What might...
History: V is a 52-year old woman who just underwent surgery that resulted in the need to transfuse two units of red blood cells. V has three children and has reported to have been transfused twice in...
History:
V is a 52-year old woman who just underwent surgery that
resulted in the need to transfuse two units of red blood cells. V
has three children and has reported to have been transfused twice
in the past.
6. Which of the
following antibodies remain after completion of the antibody
identification (completion of panel antigram)? (2 pts)
A.
Anti-Jsa
B.
Anti-Lub
C. Anti-e
D.
Anti-Jkb
7. Given the
patient history and all serology results, what is the best
hypothesis...
Forward Typin Anti-B Reverse T Ai cells Auti-A Anti B cells M a 48-year-old woman, presented...
Forward Typin Anti-B Reverse T Ai cells Auti-A Anti B cells M a 48-year-old woman, presented to the physician with a long history of dysfunctional uterine bleeding. She complained of chronic fatigue, hcadache, and a general malaisc. Upon admission to your institution her hemoglobin level was 7.5 mg dL. She was scheduled for an abdominal hysterectomy the following day and the surgcon requested three units of red cells to be given prior to the surgical procedure. Mbad no history of...
49. Interpret the following EBV-specific antibody results: IgM anti-VCA Negative IgG anti-VCA Positive Anti-EA Positive Anti-EBNA...
49. Interpret the following EBV-specific antibody results: IgM anti-VCA Negative IgG anti-VCA Positive Anti-EA Positive Anti-EBNA Positive 50. What is the major target of EBV? 51. When is the cold agglutinin titer increased? 52. What reagents and specimens are required for a cold agglutinin titer? 53. A newly hired third shift phlebotomist drew a specimen for a cold agglutinin and placed the uncentrifuged tube of blood in the refrigerator overnight. How would this affect the results of the test? 54....
Phenol red is another indicator commonly used to detect the presence of acid. This indicator is...
Phenol red is another indicator commonly used to detect the presence of acid. This indicator is red above pH7 and yellow below pH of 7. What would be the appearance of E. coli (gram negative rod, lactose fermenter) and S. aureus (gram positive cocci, lactose fermenter) if neutral red in MacConkey agar was replaced with phenol red?
In the above scenario, you introduce a dominant-negative dynamin CNDA into the cells. What might happen...
In the above scenario, you introduce a dominant-negative dynamin CNDA into the cells. What might happen after 10 minutes of treatment with ligand? A. The ligand and the receptor would not enter the cell and localize as dots. B. The ligand and receptor would appear as overlapping red green dots similar to control non mutant dynamin cells. C. The ligand and receptor would stay as overlapping dots even after 20 minutes of treatment. D. None of the above. E. All...
4. What are four test systems that employ low reagent phospholipid concentrations to detect LA as...
4. What are four test systems that employ low reagent phospholipid concentrations to detect LA as a result of it prolonging the clot formation time? 5. Which two of these tests are used in combination by most specialty or reference laboratories in the United States to detect or help rule out LAs? 6. Why do LAs prolong phospholipid-dependent clot formation? 7. What is the effect upon clotting time of adding excess phospholipids and repeating a true- positive LA screening test?...
29.) An immunoassay was performed in the following way: Antigen is bound to a solid support....
29.) An immunoassay was performed in the following way: Antigen is bound to a solid support. Patient serum is added and patient antibodies bind to the antigen. The well is washed and enzyme-labeled anti-immunoglobulin antibodies are added. The well is washed and enzyme substrate is added and enzyme activity is determined. Which immunoassay is described? A. latex agglutination B. competitive C. ELISA D. homogeneous 30.) Erythema migrans (EM) is the caused by tick bite: A. itchy rash B. red bull's-eye...
HDN= Hemolytic disease of the newborn 2. What test would you perform in order to identify...
HDN= Hemolytic disease of the newborn
2. What test would you perform in order to identify the antibody causing the HDN? 3. A new tech is investigating a suspected HDN case but the mother's cells keep on reacting as DAT negative. Assuming that HDN is actually occurring, what is the most likely error being made by the tech? 4. How would you change your titration testing procedure if you thought the antibody causing HDN was an IgG antibody against ABO?...
Diffraction can be used to provide a quick test of the size of red blood cells....
Diffraction can be used to provide a quick test of the size of red blood cells. Blood is smeared onto a slide, and a laser shines through the slide. The size of the cells is very consistent, so the multiple diffraction patterns overlap and produce an overall pattern that is similar to what a single cell would produce. Ideally, the diameter of a red blood cell should be between 7.5 and 8.0 μm. If a 633 nm laser shines through...
Diffraction can be used to provide a quick test of the size of red blood cells....
Diffraction can be used to provide a quick test of the size of
red blood cells. Blood is smeared onto a slide, and a laser shines
through the slide. The size of the cells is very consistent, so the
multiple diffraction patterns overlap and produce an overall
pattern that is similar to what a single cell would produce.
Ideally, the diameter of a red blood cell should be between 7.5 and
8.0 μm.
If a 633 nm laser shines through...
History:
V is a 52-year old woman who just underwent surgery that
resulted in the need to transfuse two units of red blood cells. V
has three children and has reported to have been transfused twice
in the past.
6. Which of the
following antibodies remain after completion of the antibody
identification (completion of panel antigram)? (2 pts)
A.
Anti-Jsa
B.
Anti-Lub
C. Anti-e
D.
Anti-Jkb
7. Given the
patient history and all serology results, what is the best
hypothesis...
Forward Typin Anti-B Reverse T Ai cells Auti-A Anti B cells M a 48-year-old woman, presented to the physician with a long history of dysfunctional uterine bleeding. She complained of chronic fatigue, hcadache, and a general malaisc. Upon admission to your institution her hemoglobin level was 7.5 mg dL. She was scheduled for an abdominal hysterectomy the following day and the surgcon requested three units of red cells to be given prior to the surgical procedure. Mbad no history of...
4. What are four test systems that employ low reagent phospholipid concentrations to detect LA as a result of it prolonging the clot formation time? 5. Which two of these tests are used in combination by most specialty or reference laboratories in the United States to detect or help rule out LAs? 6. Why do LAs prolong phospholipid-dependent clot formation? 7. What is the effect upon clotting time of adding excess phospholipids and repeating a true- positive LA screening test?...
29.) An immunoassay was performed in the following way: Antigen is bound to a solid support. Patient serum is added and patient antibodies bind to the antigen. The well is washed and enzyme-labeled anti-immunoglobulin antibodies are added. The well is washed and enzyme substrate is added and enzyme activity is determined. Which immunoassay is described? A. latex agglutination B. competitive C. ELISA D. homogeneous 30.) Erythema migrans (EM) is the caused by tick bite: A. itchy rash B. red bull's-eye...
HDN= Hemolytic disease of the newborn
2. What test would you perform in order to identify the antibody causing the HDN? 3. A new tech is investigating a suspected HDN case but the mother's cells keep on reacting as DAT negative. Assuming that HDN is actually occurring, what is the most likely error being made by the tech? 4. How would you change your titration testing procedure if you thought the antibody causing HDN was an IgG antibody against ABO?...
Diffraction can be used to provide a quick test of the size of
red blood cells. Blood is smeared onto a slide, and a laser shines
through the slide. The size of the cells is very consistent, so the
multiple diffraction patterns overlap and produce an overall
pattern that is similar to what a single cell would produce.
Ideally, the diameter of a red blood cell should be between 7.5 and
8.0 μm.
If a 633 nm laser shines through...
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