In the above scenario, you introduce a dominant-negative dynamin CNDA into the cells. What might happen...
In the above scenario, you introduce a dominant-negative dynamin CNDA into the cells. What might happen after 10 minutes of treatment with ligand?
A. The ligand and the receptor would not enter the cell and localize as dots.
B. The ligand and receptor would appear as overlapping red green dots similar to control non mutant dynamin cells.
C. The ligand and receptor would stay as overlapping dots even after 20 minutes of treatment.
D. None of the above.
E. All of the above.
Homework Answers
Dynamin is a protein involved in endocytosis pathway. It acts as a molecular scissor during the pinching off of the vesicles from membrane into the cells. So if dynamin is non functional by a dominant-negative mutation, then its loss of function will be observed. So in this case the ligands can bind to the receptor but the receptor-ligand complex cannot enter into the cell and will remain on the membrane.
A. The ligand and the receptor would not enter the cell and localize as dots.
is the correct answer.
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