In order to grow certain cells in culture the media numerous special growth factors that cannot...
In order to grow certain cells in culture the media numerous special growth factors that cannot remain active after heat sterilization, this media must be filtered to remove bacteria. What size should the pore on the filter be? Why?
Homework Answers
Ans) Filtration is that passsage of a liquid or gas theough a
screen like material with pores small enough to retain
microorganisms, used to sterilize heat-sensitive materials, such as
some culture media, enzymes, vaccines, and antibiotic
solutions
-High-efficiency particulate air(HEPA) removes almost all
miccroorganisms larger then .3um in diamyer for burn patients to
lower numbers of airborne microbes.
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In order to grow certain cells in culture the media numerous
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