Question

.AT&T 12:58 AM 1 0 95% 1:47:09 Exit D5. This week in lab you will observe the results of the transformation that you performed last week. If you were to observe colonies on your - GLO LB/amp plate, what could you conclude? The E. coli carried natural resistance to ampicillin. These are satellite colonies. The transformation results are valid. You forgot to add plasmid DNA to any of your transformations. D 6.

Answer 1

1. Transformation is the process of inculcating the vector containing the gene of interest into a bacterial cell by spreading 1microlitre of the inoculum on the LB agar plate in the Laminar Air Flow. The vector inserted in the plasmid cells have a ampicillin resistant gene and galactosidase gene, if the DNA of interest in inserted than it dis orients the open reading frame of the vector and thus the dye X-GAL is not able to express on the medium containing the colonies. Also the E.coli cells carry a massive quality of resistance towards ampicillin.