Question

Table 1; of absorbance vs. concentration on a graph sheet (use pencil, properly amylase from two sources as samples (unknowns) were also treated in d. The experimental set-up of Table 1, indicate whether it was derived using serial e. In Table 2, considering the original salivary amylase samples, which one of the b. Plot curve labeled graph will be noted or generate an excel-sheet): Use graph to extrapo late the unknowns and determine their concentrations; e. Salivary the same protocol and their absorbance measured (Table 2 below). Estimate and write the amounts in all the blank spaces in the two Table 2: dilutions; samples is more concentrated? Explain your answer conc. HO (mg/ml) I BSA (ml) Stock conc. Reagent Reading 1 Tube BSA Buret ABS ABS ABS (ml) 595nm 2e550nm(Average (ml) 10 0 0.35 0.2 0.3 0.4 0.5 0.5 0.65 0.75 0.95 0.25 0.4 0.45 0.60 0.7 0.90 7 3 Table 2 Unknowns ABS ABS Conc. Conc BiuretABS Reagent Reading 1 Reading 2 average (mg/ml) (mg/g) (ml)595nm@550nm Sol'n (ml) Salivary amylase 4 (Plaquemines) Salivary Amylase 1 (diluted with (North Shore) 3ml PBS buffer) 0.95 0.350.4

Answer 1

b.

c. Sal-amy-plaquem is 0.8mg/ml; mg/g cannot be calculated from the given data because original substance in grams not given. Sal amy north is 1.23mg/ml and it is more concentrated because it has been diluted four times, mg/g cannot be calculated from the given data because original substance in grams not given.

d. no, its not derived from serial dilutions because stock is 1mg/ml and the total reaction is 10ml = first tube is 0.1mg/ml………to last tube is 0.5mg/ml.

e. Sal amy north is 1.23mg/ml and it is more concentrated because it has been diluted four times,

Table 1; of absorbance vs. concentration on a graph sheet (use pencil, properly amylase from two sources as samples (unknowns) were also treated in d. The experimental set-up of Table 1, indicate whether it was derived using serial e. In Table 2, considering the original salivary amylase samples, which one of the b. Plot curve labeled graph will be noted or generate an excel-sheet): Use graph to extrapo late the unknowns and determine their concentrations; e. Salivary the same protocol and their absorbance measured (Table 2 below). Estimate and write the amounts in all the blank spaces in the two Table 2: dilutions; samples is more concentrated? Explain your answer Table I Tube BSA HO I BSA (ml) Stock conc. Reagent Reading 1 Buret ABS ABS Average) (mg/ml) @595nm 2e550nm 1mg/ml (ml (ml) 10 0 0.2 0.3 0.4 0.5 0.35 0.4 0.5 0.65 0.75 0.95 0.4 0.45 0.60 0.7 0.4 0.475 0.625 0.725 Table 2 Unknowns ABS Conc. Conc BiuretABS Reagent Reading 1 Reading 2 average (mg/ml) (mg/g) (ml)595nm@550nm Sol'n (ml) Salivary amylase 4 (Plaquemines) Salivary Amylase 1 (diluted with (North Shore) 3ml PBS buffer) 0.350.4 0.375 1.23