Question

Question 4 (1 point) In which of following people will the hemoglobin in their blood stream have the highest affinity for Oz? O A person resting on the beach A person running on the beach A person skiing vigorously at high altitude A person resting at high altitude Question 5 (1 point) Which of the following interactions causes a change from the R state (oxy form) to the T state (deoxy form) of hemoglobin? interactions between heme groups protonation of the R-group of His 146 on the a subunit to allow formation of a salt bridge with Lys 40 on the B subunit salt bridge formed by al-Arg141 to the carboxylate of a2Asp126 protonation of the al subunit terminal carboxyl group disrupts a salt bridge with a2 Lys 127 none of the above Question 6 (1 point) Hb Roanne has an AspG1(94)a to Glu mutation. Which of the following statements is TRUE? It has a high affinity for O2 because a hydrogen bond in the T state is broken. It has a high affinity for O2 because a hydrogen bond in the R state is broken. Cooperativity in the mutant is increased because the mutation occurs in the switch region. O The Bohr effect is diminished due to the loss of the primary binding site of the Bohr proton. The mutant has a lower affinity for O2 due to the loss of a hydrogen bond present in the T state.

Answer 1

Answer 4. (C) Amount of HB increase at high altitude resulting in more binding of oxygen to HB.

Answer 5. (A) Interaction between heme group. R- Rest stage when heme group is free to bind and T - is tense stage when heme is not free to bind.

Answer 6. (A) High affinity because it destablization of transition state from R to T state.