Question

Please do everything in task 4. Thank you!

Task 4-PREPA TION OF AN ONION ROOT TIP SLIDE the Eppendotately 5 m long and place the root tip in1.5 mL Eppendorf tube. tip to soak in the acid foe 4 tule with about 200 l. or uil the tip is complevely sulemerged, of 6 M HCL Allow the onion root 3. Transfer minutes. (MAKE SURE YOU ARE WEARING GLOVES) tip to a second Eppendorf tube. Fill the Eppendorf tube with ahout 200 , or until the tip ts 4. Place the noy's Fixative. Allow the onion root tip to soak in the fixative for 4 mimutes root t root tip, cut a 2 mm piece of the root tip. Diacard the rest. tip onto a watch glass and using a dassecting microscope, to eneure you wre cutting the conect end of the root tip out of the watch glass and place each on the middle of a micromcope lide. The side should be placed on a paper towel Place two drops 6. of carbol fustin sain on top of the 2 ㎜root tip. Let the stain oak into the toot tip for 2 minutes. minutes blot away any excess stain from the slide by using a Kimwipe. Try no to disturb the root tip 8. 9. Add 1 drop of dH O to the stained onion root E Cently place a coverslip over the onion root tip. root tip pressing straight down so as not to overlap the cells. When presing down on the slide cover the slide with a paper towel to soak up any excess stain that comes out froes between the slide and the coverslip. Be carebul not to twist the coverslip or to break the slade. The act of squashing separates the cells from each other, making the chromosomes more visible. Calculation of Tine Spen in Each Stag Stage of Cell Cycle Number of Cells FOV FOV 4 Total Interphase Prophase Metaphase Anaphase Telophase Drawings of Seages as seen in the Onioe Rock Side Stage of Mitosis Interphase Prophase 6-13 Mitosis and the Cell Cyck

Answer 1

Stage of cell cycle	Number of cells					calculation of time spent in each stage (in minutes)
	FOV1	FOV2	FOV3	FOV4	Total
Interphase	131	153	122	80	486	1044.537313
Prophase	29	22	12	23	86	184.8358209
Metaphase	6	13	9	14	42	90.26865672
Anaphase	7	8	13	6	34	73.07462687
Telophase	8	5	2	7	22	47.28358209
	Total				670

Calculation:

The equation used to calculate the total percent spent for each phase of the cell cycle is

Number of cells in a phase

————————————————— X 100 = % of Cells in phase

Total number of cells

Consider the first stage,

No of cells in interphase = 131 + 153 + 122 + 80 = 486

Total number cells = 670

therefore, percentage of cells in interphase = 486/670 x 100 = 72.5%

Similarly, the percentage is calculated for all the stages and this percentage is used in the calculation of the time taken.

Stage of cell cycle	Drawings of stages as seen in onion roots
						Interphase
Prophase
Metaphase
Anaphase
Telophase