Lab Report Questions (5 points total): In this experiment to amplify regions of 16S genes, you...
Lab Report Questions (5 points total):
In this experiment to amplify regions of 16S genes, you are performing a PCR without added DNA? Why? If an amplified PCR product is detected in this reaction, describe an appropriate course of action for how you might proceed with your experiment.
Homework Answers
- The PCR amplification is performed using 16S rRNA. The 16S gene codes for 16S rRNA which is a component of bacterial ribosome. Hence DNA is not required as template for the PCR amplication (for this experiment).
- The RNA is reverse transcribed and amplified.
- Once the PCR product is detected, it is then sequenced using Sanger Sequencing.
- Once we obtain the sequence information of the 16S rRNA, we can figure out the phylogenetic relatioship using the sequence information.
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Lab Report Questions (5 points total):
In this experiment to amplify regions of 16S genes, you...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a previous experiment, you used polymerase chain reaction (PCR) to amplify a sequence that you believe to regulate expression of a gene you are studying. You will now take this purified PCR product (double stranded DNA) and ligate it into a plasmid that contains a luciferase reporter gene. If your DNA sequence is a promoter sequence, then its presence will allow for expression of the...
Now. you should be able to answer the following questions: • How the amplification will be...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
QUESTIONS Answer the following questions in the data section of your lab report: 1. Use the...
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En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
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Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
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Question 3: In a lab experiment, you are tasked with determining the electrochemical reaction that can...
Question 3: In a lab experiment, you are tasked with determining the electrochemical reaction that can occur between aqueous hydrochloric acid (HCl)) and solid magnesium metal (Mg). When the magne- sium is placed in solution, gas bubbles begin to appear around the metal and evolve from solution. (a) Write out the two half reactions that occur when the magnesium is placed in the hydrochloric acid solution. Which species is the getting oxidized? Reduced? (b) Which species is acting as the...
Hi! Can you please answer questions a and b for my organic lab report :) The...
Hi! Can you please answer questions a and b for my organic lab
report :) The pictures below the questions is my lab procedure.
Thank you
6. Analysis Questions: a) This lab utilized a new technique called reflux, and the use of a condenser tube while heating the reaction. What is the purpose of the condenser? Why not just heat the reaction in a closed vessel instead? b) A student misread the instructions and first refluxed the sodium methoxide in...
Dry Lab 1 Report Sheet The Laboratory and S Date A. Laboratory Check-In lestructor's approval B....
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Bio 121 I need to make (yeast fermentation) lab report. This is the lab experiment and...
Bio 121
I need to make (yeast fermentation) lab
report.
This is the lab experiment and results:
This is a guide to making the lab report:
General Biology BIO121 Yeast Fermentation Lab Introduction Organisms stay alive by the utilization of energy through metabolism. The energy acquiring pathways in photosynthesis convert radiant energy from the sun into the chemical bond energy of carbohydrates. This photosynthetic process is limited to the producers or autotrophs, which include plants, photosynthetic bacteria and some protists....
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
Now. you should be able to answer the following questions: • How the amplification will be done? - How you will determine your target sequence? How the amplification will be specific for certain segment? What are the requirements to carry PCR? • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGCGGGTTGACTGCTACCTTCCCGGGATGCCCAAAATTCTCGAG-3+ +3'-GATGGACGCCCAACTGACGATGGAAGGGCCCTACGGGTTTTAAGAGCTC-5'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is...
QUESTIONS Answer the following questions in the data section of your lab report: 1. Use the experience you gained from doing the experiment to answer the following: A student tested an unknown mixture that might contain any of the ions tested in this ex- periment and made the following observations: a. On the addition of 6M HCI, the solution remained colorless and no bubbles were observed. b. When 0.1M BaCl2 was added to the acidified unknown, a white precipitate was...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
Question 3: In a lab experiment, you are tasked with determining the electrochemical reaction that can occur between aqueous hydrochloric acid (HCl)) and solid magnesium metal (Mg). When the magne- sium is placed in solution, gas bubbles begin to appear around the metal and evolve from solution. (a) Write out the two half reactions that occur when the magnesium is placed in the hydrochloric acid solution. Which species is the getting oxidized? Reduced? (b) Which species is acting as the...
Hi! Can you please answer questions a and b for my organic lab
report :) The pictures below the questions is my lab procedure.
Thank you
6. Analysis Questions: a) This lab utilized a new technique called reflux, and the use of a condenser tube while heating the reaction. What is the purpose of the condenser? Why not just heat the reaction in a closed vessel instead? b) A student misread the instructions and first refluxed the sodium methoxide in...
Dry Lab 1 Report Sheet The Laboratory and S Date A. Laboratory Check-In lestructor's approval B. Laboratory Safety and Guidelines Nome Desk No. approval for completion of inside froer cover Read Laboratory Safety and Guidelines on pages 1-4 and answer the following as true or false I. Prescription glasses, which are required by law to be "safety glasses." " can be worn in place of safety goggles in the laboratory 2. Sleeveless bNouses and tank tops are nor appropriate atire...
Bio 121
I need to make (yeast fermentation) lab
report.
This is the lab experiment and results:
This is a guide to making the lab report:
General Biology BIO121 Yeast Fermentation Lab Introduction Organisms stay alive by the utilization of energy through metabolism. The energy acquiring pathways in photosynthesis convert radiant energy from the sun into the chemical bond energy of carbohydrates. This photosynthetic process is limited to the producers or autotrophs, which include plants, photosynthetic bacteria and some protists....
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