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Determine the membrane integrity, use PI combine FACS flow cytometer or use ATP measurements, which method...

Determine the membrane integrity, use PI combine FACS flow cytometer or use ATP measurements, which method is better? why??

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Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability based on the membrane permeability. It is commonly used because it can cross compromised bacterial membranes and is therefore considered to be an indicator of membrane integrity. It stains both DNA and RNA inside of dead cells or any damaged membranes. To find the cell viability, PI stain is usually coupled with a universal stain that crosses intact membranes and stains the DNA and RNA of all cells, thereby enabling to obtain total cell counts.

Inhibition of ATP synthesis resulted in loss of membrane integrity and thereby increased production of reactive oxygen species. ATP can be measured by fluorimeter or can be measured by HPLC. It is very expensive.

Propidium iodide is better than ATP measurements.

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