1. Explain why a buffer solution should be used to prepare for the fluorescein solution. -2....
Calculate the mole ratio of dianion to monoanion of fluorescein at pH 7.4 (use the Handerson-Hasselbalch equation to calculate the ratio). Calculate the absorbance ratio of the dianion to monoanion from the absorption spectrum. Can you claim that since the absorbance is proportional to the analyte concentration, the mole ratio of the two anions should be related with the ratio of the two absorbances?
3. To make a buffer solution, you can start with a solution of a known number of moles of the base form (A) and add a strong acid until you have neutralized the correct number of moles of the base to have the correct A HA ratio for the buffer you require. For the example using HF in the background information: a. How many moles of the base form (A) are required to start? HINT: The acid form (HA) will...
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REPORT SHEET Determination of the Solubility-Product Constant for a Sparingly Soluble Salt EXPERIMENT 8 A. Preparation of a Calibration Curve Initial (Cro121 0.0024 M Absorbance 5 mL Volume of 0.0024 M K Cro Total volume 1. I mL 100 mL 2. 100ML 3. 10 mL 100ml 4. 15 mL 100 ML Molar extinction coefficient for [CrO2) [Cro,2) 2.4x100M 12x1044 2.4810M 3.6810M 0.04) 2037.37 0.85 1.13 2. 3. Average molar extinction coefficient...
Convert playlist Diction QUESTIONS 1. (2 Points) Why is it essential to keep the diazonium salt at zero degrees while you are preparing the sodium 2-naphthoxide solution? 2. (2 Points) Would you need to use sodium carbonate for the diazotization of meta- nitroaniline? Explain your answer. 3. (2 Points) What would be the IUPAC name for the azo dye if you would have used p-chloroaniline instead of sulfanilic acid? Experiment 12 4. (4 Points) Calculate the theoretical yield for Orange...