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Calculate the mole ratio of dianion to monoanion of fluorescein at pH 7.4 (use the Handerson-Hasselbalch...

Calculate the mole ratio of dianion to monoanion of fluorescein at pH 7.4 (use the Handerson-Hasselbalch equation to calculate the ratio). Calculate the absorbance ratio of the dianion to monoanion from the absorption spectrum. Can you claim that since the absorbance is proportional to the analyte concentration, the mole ratio of the two anions should be related with the ratio of the two absorbances?

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