Question

LSD1 bZIP10 Label is DNA, not antibody

Increasing amounts of Lsd (LSD1 wedge) were added to tubes that contained equal amounts of bZIP10. The resulting complexes were run on a non-denaturing gel to preserve protein interactions. The gel above is a different type of technique than those we have talked about. Instead of visualizing the protein with a labeled antibody, the protein is visualized due to its interaction with labeled DNA. Thus, you can see the position of bZIP10:Lsd complex because it binds to the DNA, but you are actually seeing the DNA.

1) What does the data show?

                As more Lsd was added, the amount of bZIP10 binding to DNA was increased

                As more Lsd was added, the amount of bZIP10 binding to DNA stayed the same

                As more Lsd was added, the amount of bZIP10 binding to DNA was reduced

2) How could this data be interpreted?

                Lsd does not affect bZIP10 binding to DNA

                Lsd enhances bZIP10 binding to DNA

                Lsd decreases bZIP10 binding to DNA

The figure above shows three different images of an undiseased normal plant cell (remember from your general biology that plant cells have vacuoles). Images II and III are fluorescence images of bZIP10 that is tagged with GFP, in image II it is false colored green and in III it is false colored red. Images I and II are the same cell viewed differently. Image III is a different cell to I and II.

3) Where is bZIP10 normally found in undiseased plants?

                Cytoplasm

                Nucleus and cytoplasm

                Nucleus

Answer 1

1. The reduction in the intensity of the band shows that: As more Lsd was added, the amount of bZIP10 binding to DNA was reduced.

2. What can be interpreted from this is that, : Lsd decreases bZIP10 binding to DNA

3. Nucleus and Cytoplasm

All the Best :)