Question

1. You are working in a lab as a Bio 199 student. Your professor asks you to use a microscope to observe the interaction between two proteins. Which of the following techniques are appropriate? A. FRAP, Fluorescence recovery after photobleaching B. FRET, Fluorescence resonance energy transfer C. Proximity ligation analysis D. FRET and proximity ligation analysis E. All of the answers are correct 2. Which two organelles appear most similar when viewed under an electron microscope? A. nucleus and lysosome B. smooth ER and Golgi apparatus C. mitochondrion and plasma membrane D. peroxisome and nucleus E. peroxisome and primary cilium 3. Congratulations! You received a $10,000 grant from the National Institutes of Health to study the function of two genes encoding two different proteins in your organism of choice. You decide to generate two “knockouts", where you remove your gene of interest from the organism to study how loss of each gene alters protein trafficking. You want to make sure that your organism has as many human homologues as possible to give your results more credibility to the public. What model organism do you choose for your study? A. human B. mouse C. fly (Drosophila melanogaster) D. worm (Caenorhabditis elegans) E. yeast (Saccharomyces cerevisiae)

Answer 1

Question 1

Proximity ligation technique is a in-situ technique and  most appropriate technique uaed for the observing the interaction between proteins. Hence, option c is correct.

Question 2

The Golgi appartus and ER have membrane with many folds. The nucleus is much larger than the lysosome and peroxisome. The mitochondria is organelle with two membrane and plasma membrane is the covering of organelle. Hence, option b is correct.

Question 3

The organism like mouse, D.melanogaster, C. elegans and S. cerevisae are used as a model organism to study useful analysis. For the human , most similar organism is mouse both genetically and physiologically. Hence, option b is correct.