Please explain. Shouldn't the last one with Is be all no, because lacIs will never remove itself from the operate even if lactose is present/or absent?
1. Under what conditions will the strain genotype I+ P+ O+ Z- Y+/ I- P+ O+ Z+ Y- express beta galactosidase? A or B?
a. This strain will not express under any conditions
b. the strain will express when lactose is present, and when lactose is absent.
Please explain reasoning. I having a trouble understand partial diploid questions. Do I think of each side separately? If one is is I+ and one is I-, who wins?
2.
Homework Answers
Lac operon contains three structural genes under the regulation
of a common set of cis-acting elements.
Cis-acting elements are DNA sequences that act in sequence context.
They cannot be complemented.
Ex: Promoter and operator
Trans-acting factors are diffusible products that can act in
trans. So, they can be complemented.
Ex: Lac repressor
Lac operon is active only when lactose is present and glucose is
absent.
P- = Lac operon is inactive irrespective of the status of any other
factor.
Oc = Constitutive operator
Is = Super repressor that cannot be inactivated by lactose
In the first case,
The genomic Y+ cannot be functional due to P-.
The Z+ on the plasmid can be active only in the presence of
lactose
In the second case,
The genomic or the plasmid Z+ and Y+ cannot be functional due to Is
irrespective of the presence or absence of lactose.
Between I- and I+, I+ wins as it is a trans-acting factor.
Add Answer to:
Please explain. Shouldn't the last one with Is be all no,
because lacIs will never remove...
PLEASE note that there may be (most likely be) more than one number that satisfies the...
PLEASE note that there may be (most likely be) more than one number that satisfies the answer. For example, for the first question, #1 may not be the only answer. PLEASE LIST ALL NUMBER THAT CORRESPONDS ISP+OCZ+Y+ For this genotype, indicate whether the enzyme is indicated is produced at significant levels. 1 ?-galactosidase, no lactose present 2 ?-galactosidase, lactose present as sole carbon source 3 permease, no lactose present 4 permease, lactose present as sole carbon source To enter your...
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In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
For each genotype, with and without inducer, select whether or not a functional galactosidase or permease...
For each genotype, with and without inducer, select whether or not a functional galactosidase or permease will be present. IS indicates a super-repressor (it can’t bind to lactose/inducers). OC indicates a constitutive operon (no repressor can bind to this region of DNA). I- ; Z- ; Y- each indicate an allele that does not produce a functional protein. P- indicates a mutation in the promoter region such that RNA polymerase does not bind. Group of answer choices I+ P+ O+...
1- For each genotype, with and without inducer, select whether or not a functional galactosidase ...
1- For each genotype, with and without inducer, select whether or not a functional galactosidase or permease will be present. IS indicates a super-repressor (it can’t bind to lactose/inducers). OC indicates a constitutive operon (no repressor can bind to this region of DNA). I- ; Z- ; Y- each indicate an allele that does not produce a functional protein. P- indicates a mutation in the promoter region such that RNA polymerase does not bind. ----------------------------------------------------------------------------------------------------------------- I+ P+ OC Z+ Y+...
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Bacteria which are stable partial diploids (F’) for the lac operon are grown in the presence or absence of lactose, as indicated below. The presence (+) or absence (-) of β-galactosidase and permease expression are investigated in these cultures. Which of the following options (A-E) would be the expected results for bacteria with the genotype IS P+ O+ Z+ Y- / I+ P+ OC Z– Y+ ? β-galactosidase Permease No lactose Lactose No lactose Lactose A - + - +...
A number of mutations affect the expression of the lactose operon in E. coir. I^- a...
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The lac operon in E. coli is a well-studied gene system, and β-galactosidase (β-gal) is the...
The lac operon in E. coli is a well-studied gene system, and
β-galactosidase (β-gal) is the product of the lacZ gene. The
diploid conditions represent the addition of a plasmid carrying
different components of the lac operon. Determine if β-gal will be
generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
PLEASE EXPLAIN ANSWERS Complete the table below using to indicate if the enzyme is synthesized and...
PLEASE EXPLAIN ANSWERS
Complete the table below using to indicate if the enzyme is synthesized and to indicate if the enzyme will not be synthesized. Genotype symbols: Ilacl (the repressor gene): P=Pac (the promoter), O = laco (the operator). Z = lacZ (the B-galactosidase gene), and Y lacy (the permease gene). The minus sign accompanying the symbol (0.g. 1) indicates a mutant gene, the plus sign indicates wild-type/functional gene. O is the constituitive operator mutation (the same as O-) and...
In this problem you will explore how to solve problems involving partial diploid lac operon bacterial strains. Bacterial strains that are "partially diploid" have two copies of the lac operon because they aquired a plasmid carrying just the lac operon region. One copy of the lac operon region is on the recipient's bacterial chromosome, and the other copy is on the P plasmid that was introduced into the cell by conjugation. Partial diploid genotypes are written with the P segment...
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generated under the conditions. Assume that glucose is absent. A +
in the genotype indicates a functioning gene, while a – indicates a
loss-of-function allele. The OC is an operator mutant that cannot
bind the lacI protein. Use...
PLEASE EXPLAIN ANSWERS
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