Question

2 mocular microscope used to calculate total Because the image only one ocular to reach Both gors through eyes. 2 What is the to wat is the total magnification for all 45x, and 97 x objectives lensest e m microscope with 15 x oculars and 4%, 10% 4X, 10X, 5 Assunting that all other riables remain constant, explain wory light of shorter wavelength will produce a clearer image than light of longer wavelengths. 4 Why is wavelength the main limiting factor on limit of resolution in light microscopy This questions is asking why we cannot use light of smaller and smaller wavelenght to get a smaller limit of resolution. 5 On a given microscope, the numerical apertures of the condenser and low power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520 m. 4. What is the limit of resolution on this microscope b. Will you be able to distinguish two points that are 330 m apart as being separate, or will they blur into one?

Answer 1

Q2.

The total magnification for each lens setting on a microscope with 15x oculars are-

MT = 15*4=60x

MT = 15*10=150×

MT = 15*45=675x

MT =15*97= 1455x

Q3.

The limit of resolution is decreased as wavelength is decreased.
Hence a smaller value for D means greater clarity of the image

Q4.

a shorter wavelength will produce a clearer image, but wavelengths shorter than 360 nm are not in the visible portion of spectrum and can't be used to make an image detectable with human eyes.

Q5.
a) L = 520 / (1.25+0.25)
= 347 nm

b)
Two points 330 nm apart will blur because they are closer together than the limit of resolution.

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