Question

What is the magnification of the image in a microscope using a 20X ocular and a 100X objective?

Name and show the equation for the the limit of resolution. What happens to the lr when you use an objective with a lower NA, or light source with a shorter wavelength   

Answer 1

​​​​​Magnification of microscope = magnification of occular lenses × magnification of objective lenses = 20 × 100 = 2000

Limit of resolution = 0.61 × wavelength of light used / numerical aperture

Limit of resolution is the minimum distance between two objects so, that they can be seen as two separate objects. Resolving power is inversely proportional to limit of resolution and is the ability of a microscope to resolve two closely placed objects as two separate objects.

If the angle of numerical aperture is decreased, than limit of resolution will increase or the distance between two objects that can be resolved will also increase. So, if we want to increase resolving power of a microscope, than we need to increase numerical aperture.

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