You will be plating 80,000 cells each into two wells on your chamber slide. Each well...
You will be plating 80,000 cells each into two wells on your chamber slide. Each well takes 1 ml of media. Your cell solution has a concentration of 2 x 106 cells/ml. By what dilution factor will you need to dilution the cell solution?
Homework Answers
Initial concentration = 2 x 10^6 cells/mL
Desired concentration = 80000 cells/mL
Dilution process:
First method:
1. Take 100 uL of starting culture and mix it with 900 uL
water/buffer (Total 1000 uL)
2. From this solution, take 400 uL and add 600 uL of water/buffer
(Total 1000 uL)
Second method:
Mix 0.04 mL (40 uL) of original sample with 0.96 mL (960 uL) of
water/buffer.
Dilution factor = 0.04
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