For Experiment 5 (Part B), you are required to prepare a 1.2 mL triple staining solution...
For Experiment 5 (Part B), you are required to prepare a 1.2 mL triple staining solution of fluorescent dyes for your HeLa cells. You will use a total volume of 60 µL stock solution of MitoTracker™ (400 nM) in the in the 1.2 mL staining solution, calculate the final concentration (in nM) and select the correct answer from the options given.
a) 20 nM
b) 20 mM
c) 8 NM
d) 0.02 mM
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For Experiment 5 (Part B), you are required to prepare a 1.2 mL
triple staining solution...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
How would you prepare a 105 mL solution of 2.2% HEPES from a 250 mM stock...
How would you prepare a 105 mL solution of 2.2% HEPES from a 250 mM stock of the buffer? (MW of HEPES = 238.30) a) First, convert 250 mM HEPES to a percent concentration (%): b) Next, determine the dilution of your percent stock solution of HEPES (calculated in "a" above) to a final concentration of 2.2% with a final volume of 105 ml: c) Mix components rightarrow ___ ml of H_2O+ ___ ml of 250 mM HEPES
You need 100 mL of a 0.00057 M solution for an experiment. You are given 10...
You need 100 mL of a 0.00057 M solution for an experiment. You are given 10 mL of a 5.7 M stock solution. What is the best method to get the final volume and concentration you for trial? -It is not possible to reach that concentration with the info presented. -Use a 1.0 mL volumetric pipette and then dilute with water to 100 mL, then repeat serially until the desired concentration is reached. -Use a graduated cylinder to measure 0.1...
11 ) During your dialysis experiment you used 5 mL of a 0.1 % solution of...
11 ) During your dialysis experiment you used 5 mL of a 0.1 % solution of starch and 5 mL of 20% glucose solution. How rnany grams of each would be in the volume used? what is each solution's new % (w/v) If you consider that each was diluted into a total volume of 10 mL.? Show all work here.
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm...
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
If you want to reproduce the shuttling experiment as performed during week 3. you will need...
If you want to reproduce the shuttling experiment as performed during week 3. you will need to purchase Cycloheximide (CHX) from one of three potential suppliers. CHX has a molar mass of 281.35 g/mol and can be dissolved in DMSO, water, or EtOH but with different solubilities. You will need to use CHX at a final working concentration of 17 μg/mL in a 150 mm dish with a total media volume of 14 mL. Determine how much of the stock...
a. If you hadn’t used blocking solution what might the staining have looked like on your...
a. If you hadn’t used blocking solution what might the staining have looked like on your nitrocellulose membranes? b. If you had boiled the protein extracts prior to the experiment what effect might this have had on your results? The expression pattern for the final three cyclins is shown above. The aim of this week’s experiment is to verify how M-cyclin is varying within the synchronised tobacco BY2 cell culture. Protein levels will be assays using a dot blot immunoassay...
If you dissolve 412 mg sucrose (formula weight: 342.29 g/mol) in 60 mL of water: a. What is the concentration of sucrose in mM in µM? b. If you take 5 mL of this solution and dilute it to 250 mL, what...
If you dissolve 412 mg sucrose (formula weight: 342.29 g/mol) in 60 mL of water: a. What is the concentration of sucrose in mM in µM? b. If you take 5 mL of this solution and dilute it to 250 mL, what is the concentration of the glucose in the resulting solution in µM? c. How many nano moles of sucrose are present in 10 mL of the diluted solution?
For Part A of the Equilibrium experiment, you will be preparing several standard solutions for the...
For Part A of the Equilibrium experiment, you will be preparing several standard solutions for the Beer's Law plot Using the information given in the Worksheet for Equilibrium Lab , answer the following questions. (a) What is the initial Fe3+ concentration of all of the solutions? 1.00 xM (b) What is the initial SCN1-concentrations of Solutions #1A - #5A? Solution #1A 1#1A | 1.00 Solution #2A 1.50 Solution #3A 2.00 Solution #4A2.50 Solution #41 2.50 M Solution #54 3,00 xM...
How would you prepare a 105 mL solution of 2.2% HEPES from a 250 mM stock of the buffer? (MW of HEPES = 238.30) a) First, convert 250 mM HEPES to a percent concentration (%): b) Next, determine the dilution of your percent stock solution of HEPES (calculated in "a" above) to a final concentration of 2.2% with a final volume of 105 ml: c) Mix components rightarrow ___ ml of H_2O+ ___ ml of 250 mM HEPES
11 ) During your dialysis experiment you used 5 mL of a 0.1 % solution of starch and 5 mL of 20% glucose solution. How rnany grams of each would be in the volume used? what is each solution's new % (w/v) If you consider that each was diluted into a total volume of 10 mL.? Show all work here.
Experiment # Starting Substrate Concentration Actual Substrate Concentration Substrate Added (ml) Buffer Added (ml) 1.5 mm 1.5 1.0 mm Nm 1.0 0.5 mm 0.50 0.250 mm 0.250 0.125 mm 0.120 1.38 0.060 MM 0.060 1.44 0.030 mm 0.030 1.47 0.015 mm 0.015 1.485 *Actual substrate concentration is calculated by taking the starting substrate concentration into the final volume of each reaction after enzyme has been added. Note that the total volume for each reaction prior to the addition of enzyme...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
For Part A of the Equilibrium experiment, you will be preparing several standard solutions for the Beer's Law plot Using the information given in the Worksheet for Equilibrium Lab , answer the following questions. (a) What is the initial Fe3+ concentration of all of the solutions? 1.00 xM (b) What is the initial SCN1-concentrations of Solutions #1A - #5A? Solution #1A 1#1A | 1.00 Solution #2A 1.50 Solution #3A 2.00 Solution #4A2.50 Solution #41 2.50 M Solution #54 3,00 xM...
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