HomeworkLib
Question

If you want to reproduce the shuttling experiment as performed during week 3. you will need...

If you want to reproduce the shuttling experiment as performed during week 3. you will need to purchase Cycloheximide (CHX) from one of three potential suppliers. CHX has a molar mass of 281.35 g/mol and can be dissolved in DMSO, water, or EtOH but with different solubilities. You will need to use CHX at a final working concentration of 17 μg/mL in a 150 mm dish with a total media volume of 14 mL.

Determine how much of the stock solution purchased from each company you would need (in μL) to add to the media in the 150 mm dish to obtain the final working concentration of CHX.

Give your answers in μL using the scientific notation.

a. Company A supplies CHX dissolved in DMSO at 100 mg/ mL:

Volume needed in μL

b. Company A supplies CHX dissolved in water in 2% (w/v) solution

Volume needed in μL:

c. Company A supplies CHX dissolved in EtOH at 50 mM

Volume needed in μL:

science   chemistry   
0 0
Add a comment Improve this question Transcribed image text
Next > < Previous
Sort answers by oldest

Homework Answers

Answer #1

company » providing conath I = 100 ng/mL = = 10suglme EHX concth at final working = 17 luglme tz volume of solh needed = 14m2coneth = 50 mm = 50 xlo-3 M = 50X10-3 x 281.35 912 = 14067.5 ug/m2 Tixvi = axua VI 17 ug Ime x lume 14067.5 ugline V = 16.91

0 0
Add a comment
Know the answer?
Add Answer to:
If you want to reproduce the shuttling experiment as performed during week 3. you will need...
Your Answer:

Post as a guest

Your Name:

What's your source?

Earn Coins

Coins can be redeemed for fabulous gifts.

Log In

Sign Up

Not the answer you're looking for? Ask your own homework help question. Our experts will answer your question WITHIN MINUTES for Free.
Similar Homework Help Questions

  • PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone,...

    PLEASE SHOW ALL WORK IF POSSIBLE! THANK YOU IN ADVANCE. 1) SOC media is 2% tryptone, 0.5% yeast extract, 10 mM NaCL (F.W. 58), 2.5 mM KCl (F.W. 74.5), 10 mM MgSO4 (F.W. 246.5), 10 mM MgCl2 (F.W. 203.3), 20 mM glucose (F.W. 180.16). Determine the amounts of each to make 500 mL of SOC. 2) Determine the number of moles in 0.05 μg of 300 bp insert DNA. There are 660 g/mol-bp. What is the molar ration of insert...

  • Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock...

    please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...

  • You need 100 mL of a 0.00057 M solution for an experiment. You are given 10...

    You need 100 mL of a 0.00057 M solution for an experiment. You are given 10 mL of a 5.7 M stock solution. What is the best method to get the final volume and concentration you for trial? -It is not possible to reach that concentration with the info presented. -Use a 1.0 mL volumetric pipette and then dilute with water to 100 mL, then repeat serially until the desired concentration is reached. -Use a graduated cylinder to measure 0.1...

  • 1) You need to make an aqueous solution of 0.209 M potassium fluoride for an experiment...

    1) You need to make an aqueous solution of 0.209 M potassium fluoride for an experiment in lab, using a 300 mL volumetric flask. How much solid potassium fluoride should you add?      grams 2)How many milliliters of an aqueous solution of 0.244 M zinc acetate is needed to obtain 11.1 grams of the salt?      mL 3) In the laboratory you dissolve 13.9 g of copper(II) chloride in a volumetric flask and add water to a total volume of 125 ....

  • Question 3: You want to prepare a fruit punch by mixing your apple juice with some...

    Question 3: You want to prepare a fruit punch by mixing your apple juice with some pineapple juice and water. 1. You want the punch to contain 40 mg of glucose from the apple juice. Which volume of apple juice should you measure? Show your calculations below. 2. You now want to add water to bring the volume to a final volume of 50 mL. How much water do you need to add? Show your calculations below. 3. You are...

  • You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...

    You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below. Sample Protein Concentration mg/mL Protein Sample (microliter) Water (microliter) 4X Sample Buffer (microliter) Total Sample Volume (microliter) Total Lysate (-) 1 mg/mL 20 E1 0.07 mg/mL 20 E2 0.15 mg/mL 20 E3 0.2 mg/mL 20

  • 1. You need to make an aqueous solution of 0.165 M barium acetate for an experiment...

    1. You need to make an aqueous solution of 0.165 M barium acetate for an experiment in lab, using a 250 mL volumetric flask. How much solid barium acetate should you add? _______grams 2. How many milliliters of an aqueous solution of 0.200 M chromium(III) sulfate is needed to obtain 7.17 grams of the salt? ________ mL 3. You wish to prepare 229 grams of 22.8 % Ba(CH3COO)2. You will need _______ grams of barium acetate and _______ mL of...

  • You find yourself setting up another Western blot, however. today you are preparing the samples t...

    You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...

  • Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml...

    Need the answers to all please :) 1) You recently purchased a new mitogen at 1mg/1ml (stock) and then prepared 1:100 dilutions as a working solution (tube A). The company also suggest verifying the optimal dilution for your experiment by doing serial (twofold) dilutions. Describe how you would prepare 500microliters of working solution (tube A). What volume of inhibitor will you add in microliter? 2) You would like to optimize the working concentration of the inhibitor, and therefore perform two-fold...

  • Go Design a Poll GT Do You Need to Gro... E FAO 3 Check Your Email...

    Go Design a Poll GT Do You Need to Gro... E FAO 3 Check Your Email Check Your Email Check Your Email 1/1 Name CHEMISTRY 1110L LAB REPORT: PROPERTIES OF SOLUTIONS Partner Desk # Part One (Do calculations on the back of this sheet, prior to lab! 1. In order to prepare 50.0 mL of 0.100 M NaOH you will add me of 100 M NaOH to m of water 2. In order to prepare 50.0 mL of 0.200 M...

ADVERTISEMENT
Free Homework Help App
Download From Google Play
Scan Your Homework
to Get Instant Free Answers
Need Online Homework Help?
Ask a Question
Get Answers For Free
Most questions answered within 3 hours.
ADVERTISEMENT
ADVERTISEMENT