You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...
- You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.
|
Sample |
Protein Concentration mg/mL |
Protein Sample (microliter) |
Water (microliter) |
4X Sample Buffer (microliter) |
Total Sample Volume (microliter) |
|
Total Lysate (-) |
1 mg/mL |
20 |
|||
|
E1 |
0.07 mg/mL |
20 |
|||
|
E2 |
0.15 mg/mL |
20 |
|||
|
E3 |
0.2 mg/mL |
20 |
Homework Answers
Add Answer to:
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml f...
a) You want to load 15 ug of protein in 10 uL into one of the...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
You are given a protein solution with a concentration of 0.15 mg/ml. We need 0.1 µg...
You are given a protein solution with a concentration of 0.15 mg/ml. We need 0.1 µg for an experiment. What volume of the protein solution do we need?
You have a stock solution that contains 24 mg/ml of protein. You want to make a...
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to...
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Need help please SOLUTION MAKING in water. Calculate the amount of NaCl you need to weigh...
Need help please
SOLUTION MAKING in water. Calculate the amount of NaCl you need to weigh out. 1 (a). You need 500mL of 15% (w/v) NaCl 1(b) What approximate volume of water would you dissolve the NaCl in? Please give a range of volumes. 1(c). Assuming that adding the NaCI to your original volume of water caused the volume to increase by 50mL before you added additional water to reach your final volume, what is the DENSITY of your final...
You find yourself setting up another Western blot, however. today you are preparing the samples t...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
lab question 1. What is the basis of the different purification methods? 2. What are some...
lab question 1. What is the basis of the different purification methods? 2. What are some of the factors the might have interfered with your results? 3. How might you improve the process to increase the yield and purity? lab process E. coli BL21 (DE3) cells were transformed with the pET Topo-1521 vector containing a reading frame encoding the green fluorescent protein (GFP). Cells were cultured in M9ZB media at 37°C until the absorbance at 600 nm reached 0.7, at...
A technologist is ask to prepare 100 mL of a 5% (v/v) concentration of sodium hypochloride....
A technologist is ask to prepare 100 mL of a 5% (v/v) concentration of sodium hypochloride. How much solute should they add to the concentrated sodium hypochloride? A CLS needs to make a 1 to 10 dilution with a total volume of 100.0 mu L. How many mu L of patient serum must be used to make this dilution? Consider if you wanted to make 1.00 L of a 0.200 M solution of Na3PO4 (163.94 g). How many grams of....
How do you prepare 500 ml of 30% v/v ethanol? How do you prepare 225 ml...
How do you prepare 500 ml of 30% v/v ethanol? How do you prepare 225 ml of 25% w/v NaCl? You have a 20 ml of a 10% w/v solution of ammonium sulfate. How many grams of ammonium sulfate are in this solution? You want to run a cesium chloride gradient to purify a sample of DNA in an ultracentrifuge. You wish to fill a 15 ml test tube with five equal layers of 5%, 10%, 15%, 20%, and 25%...
QUESTION 6 The body uses urea, H2NCONH2, to conduct waste nitrogen from protein decomposition out of...
QUESTION 6 The body uses urea, H2NCONH2, to conduct waste nitrogen from protein decomposition out of the body.Calculate the molar mass of urea and determine the number of moles of nitrogen there are in 12.6 g of urea. The molar masses of the elements are as follows: N = 14.01, C = 12.01, O = 16.00, and H = 1.008 (all g/mol) a. 0.42 mol N b. 0.21 mol N c. 0.54 mol N d. 0.47 mol N e. None...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
Need help please
SOLUTION MAKING in water. Calculate the amount of NaCl you need to weigh out. 1 (a). You need 500mL of 15% (w/v) NaCl 1(b) What approximate volume of water would you dissolve the NaCl in? Please give a range of volumes. 1(c). Assuming that adding the NaCI to your original volume of water caused the volume to increase by 50mL before you added additional water to reach your final volume, what is the DENSITY of your final...
You find yourself setting up another Western blot, however. today you are preparing the samples to load onto the SDS-PAGE gel. You are using a 4-20%SDS-PAGE gel that can hold a maximum of 30 μしper 1 You remember from you so decided to prepare each sample with a final volume of 25 uL. However, no one is perfect at pipetting, so make sure each sample is made at 1.5X the total volume needed per well. The protein you are interested...
A technologist is ask to prepare 100 mL of a 5% (v/v) concentration of sodium hypochloride. How much solute should they add to the concentrated sodium hypochloride? A CLS needs to make a 1 to 10 dilution with a total volume of 100.0 mu L. How many mu L of patient serum must be used to make this dilution? Consider if you wanted to make 1.00 L of a 0.200 M solution of Na3PO4 (163.94 g). How many grams of....
Most questions answered within 3 hours.
-
Where is the error in this code sequence?
String s1 = "Hello";
String s2 = "ello";...
asked 11 months ago -
Financial data for Joel de Paris, Inc., for last year
follow:
Joel de Paris, Inc.
Balance...
asked 11 months ago -
Consider this reaction:
Al2(SO4)3 (aq)+ BaCl3
(aq) Al2Cl6 (aq)- +
3BaSO4(s) . What is the...
asked 11 months ago -
Suppose that Savneet is considering increasing her
recent random sample from 20 car rentals to 40...
asked 11 months ago -
Trucks arrive at an unloading terminal at an average rate of 120
per hour.
Trucks arrive...
asked 11 months ago -
Why are methanol and ethanol completely soluble in water while
octanol is not very little soluble....
asked 11 months ago -
A facilities manager at a university reads in a research report
that the mean amount of...
asked 11 months ago -
When the CuSO4 is rehydrated by adding water to the anhydrous
compound, is this an endothermic...
asked 11 months ago -
A ray of sunlight is passing from diamond into crown glass; the
angle of incidence is...
asked 11 months ago -
A block of mass 0.249 kg is placed on top of a light, vertical
spring of...
asked 11 months ago -
how do the kidneys compensate in the presences of acidosis
a) trigger hyperventilate
b) reserve acid...
asked 11 months ago -
Question 501 pts
The rental rate of capital to the firm increases. Which of the
following...
asked 11 months ago