You are given a protein solution with a concentration of 0.15 mg/ml.
We need 0.1 µg for an experiment. What volume of the protein solution do we need?
You are given a protein solution with a concentration of 0.15 mg/ml. We need 0.1 µg...
You are given a protein solution with a concentration of 0.15 mg/ml. v. Suppose that we want to prepare 100 microliters of 10 micrograms/microliters solution. How much of H2O and protein stock do we need to add to obtain the target concentration and volume? (THE CONCENTRATION IS NOT GREATER IN THE QUESTION, YOU NEED TO CONVERT IT TO MICROGRAMS/MICROLITERS.... THEN IT SHOULD MAKE SENSE) ****IF YOU ARE GOING TO WRITE THIS ANSWER OUT WITH PEN AND PAPER PLEASE WRITE IN...
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below. Sample Protein Concentration mg/mL Protein Sample (microliter) Water (microliter) 4X Sample Buffer (microliter) Total Sample Volume (microliter) Total Lysate (-) 1 mg/mL 20 E1 0.07 mg/mL 20 E2 0.15 mg/mL 20 E3 0.2 mg/mL 20
A given protein solution has a concentration of 5 mg/mL, and the molecular mass of the protein is 25000 Da. (A) What is the molarity [M] of the solution? (B) What volume is needed to make 150 µL at 100 µM?
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
The absorbance (A) of the ibuprofen solution is concentration-dependent (c, µg / ml) as follows: A = 0.002391 · c - 0.003. a) What is the concentration of the solution when the absorbance is 0.735? b) What is the absorbance at 0.593 mg / ml solution?
You have a stock solution that contains 24 mg/ml of protein. You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you would make your standards by filling in the chart below. Std 1 Std 2 Std 3 Std 4 concentration (mg/ml) 12 6 3...
If a person’s blood cortisol concentration is 10 µg/mL, and 10 mg of cortisol can be excreted from the body in a 24-hour period, what volume of blood plasma in mL was “cleared” during this amount of time?
You need 100 mL of a 0.00057 M solution for an experiment. You are given 10 mL of a 5.7 M stock solution. What is the best method to get the final volume and concentration you for trial? -It is not possible to reach that concentration with the info presented. -Use a 1.0 mL volumetric pipette and then dilute with water to 100 mL, then repeat serially until the desired concentration is reached. -Use a graduated cylinder to measure 0.1...
ncetoi paper) 1. BSA stock solution A has a concentration of 3.50 mg/mL. 1.00 mL of stock solution A is diluted to a final volume of 5.00 mL to prepare solution B. 1.00 mL of solution B is diluted to a final volume of 5.00 mL to prepare solution C. 1.00 mL of solution C is diluted to a final volume of 10.00 mL to prepare solution D. Calculate the concentrations of solutions E, C, and D. Show your work....
How do protein concentration ranges compare with the DNA value? (DNA] of 50 µg/ml give an A260 of 1.0 Can you explain the difference?