Question

Predict the effects of the following on the flux through the listed pathways. Mark whether the rate of each will increase, decrease, or remain the same. If liver and muscle do not have the same pattern, indicate both in the box and mark them with with L or M

Lt illEtaUUllt in this pools in this pathway The higher the value of AG for a step, the faster the rate of the reaction of that step The likeliest steps to be regulated in this pathway are the first and last steps 2. (2.0 points) Predict the effects of the following on the flux through the listed pathways Mark whether the rate of each will increase decrease or remain the same (0). If liver and muscle do not have the same pattern indicate both in the box and mark them with L (liver) or M (muscle). Alteration Flux through Flux through Flux Flux through Flux through glycolytic Krebs cycle through Gluconeogenesis Glycogenesis pathway Example: organism is dead Have not eaten for 6 hours Anapieurotic generation of CAA is needed Cell is performing a variety of biosynthetic reactions and also has high AMP levels A release of adrenaline occurs 3. (1.5 points) Citrate synthase has been well studied, and has KM values of 16 uM for acetyl-CoA and 2 uM for oxaloacetate. affinity? a. For which substrate does citrate synthase have a higher binding b. Why is this relevant in the cell?

Answer 1

Alteration	Flux through glycolytic pathway	Flux through Kreb's cycle	Flux through PPP	Flux through Gluconeogenesis	Flux through Glycogenolysis
Dead
Not eaten for 6 hrs	L -, M -	L -, M -	L -, M-	L +, M+	L -, M-
Anapleurotic generation of OAA is needed	L -, M -	L +, M +	L +, M +	L -, M -	L -, M -
Cell is performing a variety of biosynthetic reactions and also high AMP levels	L +, M +	L +, M +	L -, M -​	L -, M -​	L -, M -​
Release of adrenaline occurs	L -, M -​	L -, M -​	L -, M -​	L -, M -​	L +, M +​