Question

Draw (or arrange) only the items you need from the Lab Materials Page (picture below) in the order you would use them to make AND screen a gene library.
You will ‘draw’ the steps that use the Lab Materials in the correct order to create and screen a gene library which has the assigned gene clone. You will need to add some connecting words and phrases to make all the steps for making your library clear.

The genomic gene clone must provide a promoter sequence of your gene for the Genetic Engineering project (backstory). (Transgenic Cattle Picture)

Don’t forget to indicate:
- The sources of the DNA or the mRNA that you are using as the starting point of your gene library
- How you will screen your gene library in order to identify the bacterial colony which has your gene

LAB MATERIALS Growth plate options (choose one): DNA from mRNA from Antibodies specific for Amp (ampicillin) Amp +Arabinose Amp +Xgal PCR primers (forward and reverse) Synthesis machine Electroporation machine Hot water E. coli Bacteria bath susceptible to all antibiotics zapper!! membranes that proteins and PCR Real time nucleotides stick to NCBI Pipetting PCR machine Tapis nilotica growth homane gene, complete od database robot Plating materials: icks, pipetters, test tubes, bags, bent glass minature hockey sticks Restriction enzymes (pick to match your plasmid): Djll, GabY, Megan1, KaRa, DevoNL, MiKEt, JenNY Gene Toot Sticky end adding enzyme Gun GabY Djll KaRa Ligase DNA polymerase DevoNL PUNL1 Megan1 PGLO Amp Res JenNY LacZ JenNY Amp Res GFP DNA Reverse transcriptase Sequencing machine KaRa

Answer 1

I have underlined the material to be used and it is written in the order .

I DNA from Cryonim 2. Digest it with Devon L [ Restriction Enzyme] 3. Amplify the desired segment with the help of PCR I a) search the NCBI database and make the desired primer using PR primer synthesis machine - 6) set up the PCR reaction by Pleing PCR pipetting Robot and then add DNA polymerase es keep the PCR reaction tubes in Real Time PCR machine 4. Take the vector PUNLI and do digast it with DevONL 5. Min the digested vector PUNLI and amplified DNA segments then add Ligose , it will insent the desired Dash fragment into the vector and for Recombinant Plasmid DNA 6. Insent the to Recombinant plasmid DNA into E.coli Bacteria Suseptible to all antibiotics. Gene Librancy has been Greated , Now hells DC Saen it 3 - 7 7. Bacterial colonies are allowed to grow on growth plate Amp + xgal 8. Bacterial colonies are transferred from surface of an agay Culture plate onto membrane that proteins and Nucleotides stick to 9. Incubate the membrane with nuckic acid probe that is labelleted with a modified nucleotide and then washed to remove unhybridized probe then the membrane is visualised using a solution of Antibody especific for probe. 1. Comparing the membrane with the orignal dish and the region of hybridization, orignal group of Colonies Can, Lining be identified. RES