Question

For an assay using a standard curve, why must unknown samples and standards be assayed simultaneously?

Answer 1

In case of standard curve, the unknown samples and standards must be simultaneously assayed as this helps in making better comparison between the two samples. It helps in calculating the absorbance and by finding out the absorbance value, it helps in finding out the value of the unknown sample. As the unknown sample and standard values are assayed together it helps in making a comparison about the values. The standard curve is basically plotting the graph from the data values of the sample and hence it is important that one is aware of the amount present in the unknown sample. As the unknown sample and standard is assayed together simultaneously, it helps in drawing the graph and also extrapolating the data