What would be some reasons as to why a bacterial growth curve
would produce unexpected results? For example, an E. coli culture
increases after the stationary phase? Or why there is no
statio
nary phase? I am trying to
interpret results that I have from a lab experiment.
In Escherichia coli, some cells called persisters remain dormant during the log phase of growth and start to grow during or after the stationary phase. This could be the reason for getting unexpected results like not getting the stationary phase or increase in cell density after stationary phase.
What would be some reasons as to why a bacterial growth curve would produce unexpected results?...
this is a bacterial growth curve experiment . please explain
rhe results
Procedure: You will follow the growth of E. coli over the course of the period (3 hrs) by making direct counts of the bacterial suspension by measuring the turbidity of a sample at a given time with a spectrophotometer. The data obtained from the direct counts will be used to plot a partial growth curve. Summary: Turbidity Counts with the Spectrophotometer to measure absorbance at 600. Direct Counts...
these are my results for my bacteria growth curve of e coli.
expected results were to have a lag phase, exponential phase,
stationary phase and death phase. the first phase is declining so
doesnt resemble a lag phase, and no death phase is present. please
explain why/ what are possible reasons in the difference in growth
curve vs what i expected
OD600 readings and cell concentration Cell Concentration (cell/mL) 1000000000 157000000 93700000 52700000 53000000 100000000 -30900000 10000000 21 SOXOXO Cell...
a) During the exponential phase of bacterial growth, the lab strain of E. coli has doubling time of about 30 minutes. If you start with 10,000 cells per mL of culture, and let them grow exponentially 2 hours in a flask, what is the final density of the culture? Show and explain your calculations. b) If you plated a100μL of 1:1000 dilution that culture (after it had grown in a flask for 2 hrs) how many colonies would you expect...
1:23 h Date Laboratory Report 45) Determination of a Bacterial Growth Curve and the Generation Time of a Culture 1. Collect your data on alsorance in the following table. Inbeton Time Absorbance 600 m 083 336 SIE .769 971 150 2. Estimate the generation time for this E. coli culture by the indirect method using your growth curve graph and extrapolations from the absorbances for doubling. Be sure to put UNITS on your answers. * From growth curve (indicate what...
please use these results of a growth curve experiment to
answer the following questions
Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you...
"Three organisms were innoculated on a culture media. Results showed Escherichia coli as red colonies, no growth of Staphylococcus epidermidis, and colorless colonies of Salmonella enterica. The medium is:" a selective and differential b differential c selective d minimal e heterotrophic "A bacterium was incubated with aeration in a nutrient medium containing two carbon sources. After logarithmic growth, a lag phase was observed for 25 minutes. Then logarithmic growth at a slower rate was observed. Which substate was preferred by...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
Consider the
effect on your experimental results of the following and explain
what the effect would be and why.
a) The reaction of
BB- and OCl- turned out to be strongly
exothermic.
i don't really know
how this would change the absorbance levels over time. Is this
reaction already exotermic or is it endothermic.
results
We were unable to transcribe this imageD E F G H I J K L M N O P 1 Time (s) Absorbance In (Absorbance)...
Metabolic Pathway Engineering Problem Set 5 Engineering a Fermentation System: Fermentation of plant matter to produce ethanol for fuel is one potential method for reducing the use of fossil fuels and thus the CO2 emissions that lead to global warming. Many microorganisms can break down cellulose then ferment the glucose to ethanol. However, many potential cellulose sources, including agricultural residues and switchgrass, also contain substantial amounts of arabinose, which is not as easily fermented. Escherichia coli is capable of fermenting...
1. You are studying phage transduction and trying to deliver a kanamycin resistance gene from one strain of bacteria to another using phage. The kanR gene is FAR away from the lambda prophage in your bacterium. Choose which type of transduction should you use for your experiment and explain why. a. lambda phage, specialized transduction b. lambda phage, conjugation C. T4 phage, generalized transduction d. A different phage with mitomycin C induction 2. A pig is infected with two subtypes...