you want to make RNA in vitro, how would you do that? meaning what would you...
you want to make RNA in vitro, how would you do that? meaning what would you add to get RNA in PCR instead of DNA
Homework Answers
Answer:
RNA synthesis requires a DNA template, RNA polymerase, Nucleotide triphosphates (NTPs) and other transcription factors.
During conventional PCR, DNA Taq polymerase replicates DNA strands, when RNA polymerase is used instead with appropriate primers and NTPs, RNA can be formed.
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you want to make RNA in vitro, how would you do that? meaning
what would you...
Explain Base hydrolysis of DNA and RNA - What possible products would you see? - Which...
Explain Base hydrolysis of DNA and RNA - What possible products would you see? - Which is more readily seen and why - for PCR why do we add dNTPS?
Why do we want/need the presence of Mg2+ in a PCR but do not want it...
Why do we want/need the presence of Mg2+ in a PCR but do not want it present at all other steps of processing/analyzing a DNA sample? (2 marks) Identify and explain what generally occurs in each of the 3 main steps of a polymerase chain reaction. (6 marks) 8. Identify and explain what the 3 different PCR controls inform you about the results of a PCR. (3 marks) Highlight the 2 advantages and 3 disadvantages of using PCR. Ensure to...
what is the goal of in vitro transcription? why do we synthesise rna and what can...
what is the goal of in vitro transcription? why do we synthesise rna and what can we use that for? what is tRNA used for?
Question 1: As a researcher, you want to prevent gene expression by cleaving the product of...
Question 1: As a researcher, you want to prevent gene expression by cleaving the product of transcription. Which regulatory mechanism would you use? a, miRNA b. siRNA c. miRNA or siRNA d. proteasome e. RNA splicing ------------------------------------------------------------------------------------------------------------------- Question 1a: Which statement represents a difference between DNA replication in your cells and amplification through PCR? a. All of the above represents differences b. DNA primers are required for PCR, but not in your cells c. PCR involves differential temperature, but your...
1. How would you define geneticengineering? 2. How would you make a fly that has the...
1. How would you define geneticengineering? 2. How would you make a fly that has the HRP gene in its cells? 3. Watch Video "How to Perform Colony PCR" and then answer the below questions: https://www.youtube.com/watch?v=hoyRrowtd44 How was DNA able to move along the agarose gel and be ordered by size? What would have been needed in the PCR reaction to obtain bands for the gene that was inserted into the vector? 4. Watch video on microinjecting DNA into germline...
if you randomly synthesized RNA strands in vitro using a 1:3 mixture of C:U nucleotides, and...
if you randomly synthesized RNA strands in vitro using a 1:3 mixture of C:U nucleotides, and then translated those RNA strands in vitro, what proportion of the amino acids in the resulting polypeptide chains will be serine amino acids?
how do you determine DNA/RNA purity, how do you determine DNA concentration, how do you determine...
how do you determine DNA/RNA purity, how do you determine DNA concentration, how do you determine protein concentration, and how do you determine cell density?).
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the...
Suppose, you synthesized RNA in vitro using polynucleotide phosphorylase, 3 parts ADP and one part GDP...
Suppose, you synthesized RNA in vitro using polynucleotide phosphorylase, 3 parts ADP and one part GDP After the reaction, you isolated all RNA molecules and translated them in a cell-free translation system. Now, answer the following questions. (A) How many different amino acids do you expect in the polypeptides synthesized in the cell-free system? Name these amino acids. Use the codon dictionary available in the textbook. (B) Which amino acid would show highest frequency of incorporation in the polypeptides synthesized...
If you have plasmid DNA and want to isolate a specific gene, do you need to...
If you have plasmid DNA and want to isolate a specific gene, do you need to perform both RFLP and PCR? Or would only one of those techniques be needed?
1. How would you define geneticengineering? 2. How would you make a fly that has the HRP gene in its cells? 3. Watch Video "How to Perform Colony PCR" and then answer the below questions: https://www.youtube.com/watch?v=hoyRrowtd44 How was DNA able to move along the agarose gel and be ordered by size? What would have been needed in the PCR reaction to obtain bands for the gene that was inserted into the vector? 4. Watch video on microinjecting DNA into germline...
Suppose, you synthesized RNA in vitro using polynucleotide phosphorylase, 3 parts ADP and one part GDP After the reaction, you isolated all RNA molecules and translated them in a cell-free translation system. Now, answer the following questions. (A) How many different amino acids do you expect in the polypeptides synthesized in the cell-free system? Name these amino acids. Use the codon dictionary available in the textbook. (B) Which amino acid would show highest frequency of incorporation in the polypeptides synthesized...
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