Question

Place the items below in the correct order by dragging each item box and placing it in the correct order in the empty space that is provided.

Answer 1

Answer: This is the correct order from first step to the last.

1	DNA gyrase removes the DNA supercoils
2	DNA helicase unwinds the DNA double helix
3	Binding proteins stabilize the unwound double helix
4	Primase generates a RNA primer that binds to the origin
5	DNA pol III adds complementary nucleotides
6	DNA pol I replaces the RNA primer
7	DNA ligase seals any nicks in the DNA
8	Topoisomerase IV separates linked chromosomes

Explanation:

1.) DNA before replication is in supercoiled state. DNA gyrase removes the DNA from supercoiling state.

2.) The DNA is is double stranded helix. So, it needs to be unwound in order to initiate the replication. DNA helicase unwinds this double stranded DNA.

3.) Single stranded binding proteins does not allow the bases again to anneal and also prevents degradation. This was it stabilizes the DNA in unwinded stage.

4.) Primase lays down the RNA nucleotides so that the DNA polymerization can begin.

5.) DNA polymerase III is responsible for the addition of DNA nucleotides in the 5' to 3' direction in the growing strand.

6.) DNA polymerase I remove the RNA primer and adds the DNA nucleotides instead.

7.) DNA nucleotides added have some gaps in between. Ligase fills the gap in the DNA.

8.) As there are multiple forks during the DNA replication, the resulting chromosomes will be linked together. So an enzyme is required to separate linked chromosomes. Topoisomerase IV enzyme is responsible for this.