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7. In contrast, when investigators are trying to manually delete a specific gene for the first...

7. In contrast, when investigators are trying to manually delete a specific gene for the first time, they introduce (transform) their gene-deleting construct into a diploid strain. What is their rationale in this case?

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Answer #1

These deleted gene is cloned in a vector which after transformation enter into cell and cause homologous recombination to replace gene of interest from genome of wild type cells which could be easily selected over certain selection medium, where only hologous recombination cells are selected.

Hope it's clear..thanks.

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