Question

oligono rims (a Why is Fluid Thioglycollate Medium well-adapted for the growth of obligate anaerobes? ds svo ulla dole geab 7605 s lo noisduon 1911 S A) The sodium thiogycollate removes the oxygen that would inhibit the growth of the anaerobe. dossis v aste A B) Anaerobes are fastidious and require the nutrients found in FTM. C) The resazurin promotes growth of anaerobes. donec D) FTM suppresses the growth of aerobes allowing anaerobes to flourish. 6. Although Fluid Thiogycollate Medium allows for the growth of microbes in all aerotolerance categories, it is more commonly used for the growth of anaerobes and microaerophiles because A) it contains the proper nutrients for these organisms. A B) it contains ingredients that prevent oxygen from becoming equally diffused throughout the whole tube. the amount of carbon dioxide in the tube is very high.donisia D) the amount of agar in the tube is very low. Page 2 NO2 GAS MOT MACOFO OFF Additional Digits of Seven-Digit Profile hat each test has a value of values for each gr

Answer 1

ANS:5.OPTION a) is correct.The Fluid Thiogllycolate medium well adapted for the growth of the obligate anaerobes

Reason: Because Sodium Thoiglycolate in the medium consumes oxygen and allows the growth of obligate anaerobes.resazurine is the redox dye that determine the oxygen concentration at given level it is give pink colour in the presence of oxygen.Sodium Thiogllycate bind with oxygen in the broth and produce range of oxygen concentration in the media along its depth so,obligate anaerobes grow at the bottom of the test tube where oxygen concentration is very less.

ANS6:-OPTION b) is correct,Fluid Thioglycolate in the medium reacts with oxygen and produce the different range of oxygen concentration from top to bottom in the test tube .areotolerlants organisms no need of oxygen as they metabolise energy anaerobically and sodium thioglycolate used the oxygen and allow the growth of different microbes according to the oxygen concentration.

ANS7-:OPTION is a) correct,gas generator enevelop creats vacuum in the anaerobic jar for the anaerobic condition essential for the growth of bacteria.

ANS8;-OPTION A) Anaerobic jar is used to culture the anaerobic bacteria

REASON :Because anaerobic bacteria are unable to grow in the presence of oxygen.

ANS9:- OPTION a),TRUE motility is differ from the Brownian motion

REASON:-true motility is the ability of the organism in which they move freely in some distance.Brownian movement exhibit by the liquid or gases molecules,in which small ,invisible molecules vibrate the medium.

ANS10-OPTION b )is correct,Wet mount preparation of bacteria more difficult to see under the microscope than the gram positive bacteria because cell not stain with contrast with the slide ,stain provides color to the cell in this way their apperance recognise easily and in wet preparation slide cell easily move here and there ,some are stick here there and without staining not seen properly.