Question

In a sample, you used 0.5 g of wheat germ in 5 ml of enzyme extraction buffer (total volume of 5.5 mL). After homogenization and centrifugation,you used a 250 µl aliquot of the wheat germ extract to determine the amount of acid phosphatase present. You found that 3.2 µg of acid phosphatase was present in the 250 µl aliquot.
First off, what is the concentration of acid phosphatase in your extract?

1. 12.8 ug/uL
2. 0.0128 ug/uL
3. 0.00058 ug/uL
4. 0.5 ug/mL

How many micrograms of acid phosphatase are present in a gram of wheat germ from your extract?

1. A. 128
2. B. 107
3. C. 140.8
4. D. 0.1408

Answer 1

concentration is B-0.0128 microgram/microlitre.

micrograms of acid phosphatase is A-128

3.0 M 여 aced phosphatase "in present on

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