Question

1. Wild type GFP has an emission max of 509nm and an excitation max of 395 nm. What are your microscope settings? You can respond once 1.0 0.81 Relative intensity 0.61 M 0.4 0.24 0.0L 300 400 500 alalanath inmol 600

Answer 1

Accoreing tobresearchers, RGFP signal can be weak and hard to detect, especially when expressed at low levels, fluorescent cells are usually observed using a laser scanning confocal microscope or similar instrument.

A weak signal thus precludes use in a microplate spectrofluorometer, which would allow increased throughput.

The SPECTRAmax GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics that permit experimental determinations at low-level detection limits.

This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein (GFP) in a 96-well microplate using the SPECTRAmax GEMINI-XS spectrofluorometer.